PXD053644-1
PXD053644 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phenotypic screening against P. falciparum under physiologically relevant nutrients levels reveals novel chemical diversity and highlights the importance of the PSAC channel for parasite survival. |
| Description | Microenvironment mapping was used to identify the binding targets of two small molecules in Plasmodium Falciparum cells. In the method, small molecule - photocatalyst conjugates sensitize biotinylation of proteins binding the small molecules, which were reduced, alkylated with iodoacetamide, and immunoprecipitated using streptavidin beads. Labeling was subject to offcompete using a large excess of the free small molecule. On-bead tryptic digestion yielded peptides, which were subjected to DDA-PASEF MS measurement using a timsTOF Pro 2. CLAG3 was significantly enriched by labeling by both molecules. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-07-05 |
| AnnouncementXML | Submission_2024-07-05_06:50:54.371.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jacob Geri |
| SpeciesList | scientific name: Plasmodium falciparum; common name: malaria parasite P. falciparum; NCBI TaxID: 5833; |
| ModificationList | Carbamidomethyl |
| Instrument | timsTOF Pro 2 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-04 09:34:59 | ID requested | |
| ⏵ 1 | 2024-07-05 06:50:54 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Proteomics, microenvironment mapping, target identification, TIMSTOF |
Contact List
| Jacob Geri | |
|---|---|
| contact affiliation | Weill Cornell Medicine |
| contact email | jag4016@med.cornell.edu |
| lab head | |
| Jacob Geri | |
| contact affiliation | Weill Cornell Medicine |
| contact email | jag4016@med.cornell.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095249/ |
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