PXD053621-1

PXD053621 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling reveals new functional relationships between meiotic recombination proteins in S. cerevisiae
Description	Several protein ensembles facilitate meiotic crossover recombination and the associated process of synaptonemal complex (SC) assembly during meiosis. We have employed proximity labeling as a phenotyping tool to investigate functional requirements for spatial relationships between meiotic recombination and SC proteins in S. cerevisiae, and to gain deeper insight into the molecular deficits of crossover-deficient meiotic mutants. We find that recombination initiation and synaptonemal complex structures are dispensable for proximity labeling of the Zip3 E3 ligase by components of the ZZS ensemble (Zip2, Zip4 and Spo16) but enzymes associated with early steps in recombination are required for Zip3 proximity labeling by MutSg, consistent with the possibility that MutSg joins Zip3 only after a specific recombination intermediate has been generated. Proximity labeling furthermore suggests that a key defect of crossover-defective, SC-proficient zip1 separation-of-function mutants is a failure to assemble an early recombination ensemble where MutSg can properly engage Zip3. We also find that the SC structural protein Ecm11 is proximity labeled by ZZS proteins in a Zip4-dependent and Zip1-independent manner, but by Zip3 and Msh4 at least in part via a distinct pathway that relies on Zip1. Finally, streptavidin pulldown followed by mass spectrometry on eleven proximity labeling strains uncovered shared proximity targets of SC and crossover-associated proteins, some of which have not yet been implicated in meiotic recombination or SC formation highlighting the potential of proximity labeling as a discovery tool.
HostingRepository	PRIDE
AnnounceDate	2024-10-02
AnnouncementXML	Submission_2024-10-02_12:29:53.406.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeremy Balsbaugh
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 18:03:20	ID requested
⏵ 1	2024-10-02 12:29:53	announced
2	2024-10-22 07:00:30	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics, recombination, yeast, meiosis,Proximity labeling, synaptonemal complex, label free

Jeremy Balsbaugh
contact affiliation	Proteomics & Metabolomics Facility, University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliation	University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053621
     1. Label: PRIDE project
     2. Name: Proximity labeling reveals new functional relationships between meiotic recombination proteins in S. cerevisiae