PXD053608-1

PXD053608 is an original dataset announced via ProteomeXchange.

Title	PLEKHA4 knockdown induces apoptosis in melanoma cells through the MAPK and cMyc signaling pathways
Description	Melanoma, originating from the malignant transformation of melanocytes, is the deadliest form of skin cancer. The incidence of primary cutaneous melanoma is increasing, with about 132,000 new cases annually. While early-stage melanoma can often be treated successfully with surgery, advanced melanoma remains challenging, necessitating new therapeutic targets. The MAPK pathway, particularly involving MEK and ERK, is critical in melanoma progression. PLEKHA4, a protein involved in cancer biology, has been identified as a key factor in melanoma cell proliferation and progression. Our study investigated the role of PLEKHA4 in regulating MAPK signaling and its impact on drug sensitivity in melanoma. Proteomic analysis, cell culture experiments, and various molecular techniques, including immunoblotting and quantitative proteomics, were employed to elucidate PLEKHA4’s function. Results indicates that PLEKHA4 is overexpressed in melanoma, promoting cell proliferation. PLEKHA4 knockdown induces apoptosis through the MAPK and cMyc pathway. enhances cMyc ubiquitination and degradation, suggesting a potential mechanism for its role in melanoma. These findings highlight PLEKHA4 as a significant player in melanoma progression and a potential target for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-01-22
AnnouncementXML	Submission_2025-01-21_22:10:42.549.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuyang Yue
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 07:35:55	ID requested
⏵ 1	2025-01-21 22:10:43	announced

Yue Y, An G, Cao S, Li X, Du L, Xu D, Jin T, Liu L, catenin signaling. Mol Med Rep, 31(1):(2025) [pubmed]

10.3892/mmr.2024.13395;

submitter keyword: cMyc, MAPK,PLEKHA4, apoptosis

Dongyuan Xu
contact affiliation	Key Laboratory of Cellular Function and Pharmacology of Jilin Province, Yanbian University,
contact email	dyxu@ybu.edu.cn
lab head
Yuyang Yue
contact affiliation	Yanbian University Hospital
contact email	yyytundra@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053608
     1. Label: PRIDE project
     2. Name: PLEKHA4 knockdown induces apoptosis in melanoma cells through the MAPK and cMyc signaling pathways