PXD053607-2

PXD053607 is an original dataset announced via ProteomeXchange.

Title	A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity
Description	Outer mitochondrial membrane (OMM) proteins communicate with the cytosol and other organelles including the endoplasmic reticulum (ER). This communication is important in thermogenic adipocytes to increase energy expenditure that controls body temperature and weight. However, the regulatory mechanisms of OMM protein insertion are poorly understood. Herein, stress-induced cytosolic chaperone PPID (peptidyl-prolyl isomerase D/cyclophilin 40/Cyp40) drives OMM insertion of the mitochondrial import receptor TOM70 regulating body temperature and weight in obese mice, and respiratory/thermogenic function in brown adipocytes. PPID PPIase activity and C-terminal tetratricopeptide repeats (TPR), which show specificity towards TOM70 core and C-tail domains, facilitate OMM insertion. Our results provide an unprecedented role for ER-stress-activated chaperones in controlling energy metabolism through a selective OMM protein insertion mechanism with implications in adaptation to cold temperatures and high-calorie diets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:33.669.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Schmoker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-03 07:18:27	ID requested
1	2024-10-03 05:06:57	announced
⏵ 2	2024-10-22 07:00:34	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: XLMS, TOM70, CLMS, crosslinking

Pere Puigserver
contact affiliation	Department of Cell Biology, Harvard Medical School Department of Cancer Biology, Dana-Farber Cancer Institute
contact email	pere_puigserver@dfci.harvard.edu
lab head
Anna Schmoker
contact affiliation	Dartmouth Cancer Center, Dartmouth Colleg
contact email	aschmoker@crystal.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053607

PRIDE project URI

• PRIDE
  1. PXD053607
     1. Label: PRIDE project
     2. Name: A chaperone-assisted mechanism of outer mitochondrial membrane TOM70 insertion protective against diet-induced obesity