PXD053556-1

PXD053556 is an original dataset announced via ProteomeXchange.

Title	Desulfuromonas acetexigens full proteome
Description	Desulfuromonas acetexigens is an anaerobic, Gram-negative bacterium capable of chemoorganotrophic growth on acetate as the energy and carbon source with elemental sulfur, fumarate, or malate as electron acceptors. D. acetexigens, first isolated from anoxic freshwater sediments, is a particularly interesting organism for studying extracellular electron transfer due to the ability to produce high current in a short amount of time. This ability could be attributed to several possible pathways, such as the porin-cytochrome-mediated or metal-reducing pathways, extensively studied in other organisms, such as Geobacter sulfurreducens and Schewanella oneidensis. Understanding the fundamentals of EET mechanisms coupled with the ongoing identification of novel organisms with EET capabilities is crucial for the advancement and optimization of microbial electrochemical technologies. These technologies hold remarkable potential for groundbreaking applications in bioremediation, biosensor development, and resource recovery operations. At present, a primary challenge holding back the scale-up and commercial viability of these applications is the relatively low efficiency of EET processes. Earlier sequenced genome allowed us to find genes related to the respective pathways, suggesting that D. acetexigens may use different pathways depending on the conditions. A previous study revealed that the redox potentials of EET-related proteins could be in the range from -0.5 V to +0.15 V vs. Ag/AgCl2. However, assessing whether the pathways are functional without comprehensive proteomics analysis is challenging. Here, we compared protein expression in biofilms grown under four different applied potentials to fumarate-grown planktonic culture using LC/MS2 operating in DIA mode. Further, we compared D. acetexigens proteins against G. sulfurreducens using PAW BLAST to better understand the function of less characterized proteins.
HostingRepository	PRIDE
AnnounceDate	2025-11-30
AnnouncementXML	Submission_2025-11-30_03:54:24.109.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maksim Sysoev
SpeciesList	scientific name: Desulfuromonas acetexigens; NCBI TaxID: NEWT:38815;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-02 01:18:35	ID requested
⏵ 1	2025-11-30 03:54:24	announced

10.1016/J.BITEB.2025.102448;

submitter keyword: MEC, LC-MS,Desulfuromonas acetexigens, proteome

Pascal Elias Saikaly
contact affiliation	MAPTech Lab, King Abdullah University of Science and Technology (KAUST), Kingdom of Saudi Arabia
contact email	pascal.saikaly@kaust.edu.sa
lab head
Maksim Sysoev
contact affiliation	King Abdullah University of Science and Technology
contact email	maksim.sysoev@kaust.edu.sa
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD053556

PRIDE project URI

• PRIDE
  1. PXD053556
     1. Label: PRIDE project
     2. Name: Desulfuromonas acetexigens full proteome