PXD053554-1

PXD053554 is an original dataset announced via ProteomeXchange.

Title	Phospho-tyrosine proteomic analysis in Ptprd-meB+/– mice
Description	PTPδ, encoded by PTPRD, is implicated in various neurological, psychiatric, and neurodevelopmental disorders, but the underlying mechanisms remain unclear. PTPδ trans-synaptically interacts with multiple postsynaptic adhesion molecules, which involves its extracellular alternatively spliced mini-exons, meA and meB. While PTPδ-meA functions have been studied in vivo, PTPδ-meB has not been studied. Here, we report that, unlike homozygous PTPδ-meA-mutant mice, homozygous PTPδ-meB-mutant (Ptprd-meB–/–) mice show markedly reduced early postnatal survival. Heterozygous Ptprd-meB+/– male mice show behavioral abnormalities and decreased excitatory synaptic density and transmission in dentate gyrus granule cells (DG-GCs). Proteomic analyses identify decreased postsynaptic density levels of IL1RAP, a known trans-synaptic partner of meB-containing PTPδ. Accordingly, IL1RAP-mutant mice show decreased excitatory synaptic transmission in DG-GCs. Ptprd-meB+/– DG interneurons with minimal IL1RAP expression show increased excitatory synaptic density and transmission. Therefore, PTPδ-meB is important for survival, synaptic, and behavioral phenotypes and regulates excitatory synapses in cell-type-specific and IL1RAP-dependent manners.
HostingRepository	PRIDE
AnnounceDate	2025-04-01
AnnouncementXML	Submission_2025-04-01_01:17:33.816.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yeji Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-01 23:13:26	ID requested
⏵ 1	2025-04-01 01:17:34	announced

Dataset with its publication pending

submitter keyword: Phospho-tyrosine proteomic analysis, Ptprd-meB, LC-MS/MS

Jin Young
contact affiliation	Korea Basic Science Institute
contact email	jinyoung@kbsi.re.kr
lab head
Yeji Yang
contact affiliation	Korea Basic Science Institute
contact email	yangyj@kbsi.re.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053554
     1. Label: PRIDE project
     2. Name: Phospho-tyrosine proteomic analysis in Ptprd-meB+/– mice