PXD053516-1

PXD053516 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	TAS1440, an LSD1 inhibitor, activates TGF-β and NOTCH signaling in neuroendocrine small-cell lung cancer
Description	NCI-H1417 cells were electrotransfected with either a pcDNA3-3×Flag-myc-empty or pcDNA3-3×Flag-myc-LSD1. Cells were treated with different LSD1 inhibitors for 2 h, harvested. Lysates were immunoprecipitation by using ANTI-Flag® M2 Affinity Agarose Gel (Cat. No.‎ A2220, Sigma-Aldrich) overnight at 4°C. After washing and eluting by 3× FLAG® peptide (Cat. No.‎ F4799, Sigma-Aldrich). Eluted samples were precipitated using acetone. The pellet was dissolved in 1× SDS sample buffer and resolved by SDS–PAGE. The Bands were excised from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Fisher Scientific). The resulting LC-MS/MS dataset was analyzed using Mascot software (version 2.5.1, Matrix Science, Boston, MA, USA). The Mascot score indicates the probability that the observed match was a random event (a Mascot score > 100 indicates that the absolute probability is <1 × 10−10).
HostingRepository	jPOST
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_08:00:06.011.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap Velos Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-29 22:51:56	ID requested
⏵ 1	2025-06-29 08:00:06	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Cancer, histone H3 competitor, insulinoma-associated protein 1,‎ lysine-specific histone demethylase 1A, NOTCH, ‎small-cell lung cancer‎, TAS1440, TGF-β

submitter keyword: Cancer, histone H3 competitor, insulinoma-associated protein 1,‎ lysine-specific histone demethylase 1A, NOTCH, ‎small-cell lung cancer‎, TAS1440, TGF-β

Contact List

Tomoaki Tanaka
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003190/