PXD053489-1

PXD053489 is an original dataset announced via ProteomeXchange.

Title	PEX14 acts as a molecular link between optineurin and the autophagic machinery to induce pexophagy
Description	Peroxisome degradation, known as pexophagy, is essential for eliminating surplus and dysfunctional peroxisomes, with dysregulation linked to various diseases. Previous research revealed that ectopic expression of optineurin (OPTN), a versatile autophagy adaptor involved in multiple autophagic pathways (e.g., mitophagy, aggrephagy, and xenophagy), triggers pexophagy in HEK-293 cells. However, the underlying mechanism remained elusive. Here, we demonstrate that OPTN-mediated pexophagy is a cell type-specific process. In addition, using proximity labeling, we identified PEX14, a peroxisomal membrane protein, as an OPTN-interacting partner. GFP-Trap analyses confirmed this interaction and revealed that PEX14 and OPTN interact through their respective coiled-coil and C-terminal ubiquitin-binding domains, independently of ubiquitin. Furthermore, our results indicate that the C-terminal half of OPTN-GFP induces pexophagy by oligomerization with endogenous OPTN. Overall, these findings propose PEX14 as a docking factor for OPTN at the peroxisomal membrane, facilitating the connection between peroxisomes and the autophagic membrane scaffold during OPTN-mediated pexophagy.
HostingRepository	PRIDE
AnnounceDate	2025-09-23
AnnouncementXML	Submission_2025-09-23_06:59:11.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Li Hongli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-28 06:04:47	ID requested
⏵ 1	2025-09-23 06:59:11	announced

10.1083/JCB.202411184;

submitter keyword: PEX14,peroxisomes, pexophagy, ubiquitin, optineurin

Marc Fransen
contact affiliation	KU Leuven, Department of Cellular and Molecular Medicine, Laboratory of Peroxisome Biology and Intracellular Communication, 3000 Leuven, Belgium.
contact email	marc.fransen@kuleuven.be
lab head
Li Hongli
contact affiliation	KU LEUVEN
contact email	hongli.li@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053489
     1. Label: PRIDE project
     2. Name: PEX14 acts as a molecular link between optineurin and the autophagic machinery to induce pexophagy