PXD053457-1

PXD053457 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Targeted Quantification of SnRK2.6 Activation Loop Phosphorylation Status at Positions 171 and 175/176 in NIL-DOG1 and dog1-1 Dry Seeds.
Description	Stable transgenic seeds expressing SnRK2.6 as an N-terminally tagged YFP fusion in two genetic backgrounds, NIL-DOG1 and dog1-1, were used as the starting biological material. The target protein was purified through immunoprecipitation from native dry seed crude extracts of both genotypes in three independent biological replicates. Samples were split and measured using both DDA and PRM-based methods. This assay aimed to investigate the influence of the dog1-1 mutation on the phosphorylation status of SnRK2.6 activation loops.
HostingRepository	jPOST
AnnounceDate	2024-06-27
AnnouncementXML	Submission_2024-06-27_04:15:59.161.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume Nee
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	instrument; instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-27 04:15:55	ID requested
⏵ 1	2024-06-27 04:15:59	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: (STY) Phosphorylation, PRM, ABA Signaling

submitter keyword: (STY) Phosphorylation, PRM, ABA Signaling

Contact List

IRIS FINKEMEIER
lab head
Guillaume Nee
contact affiliation	INSTITUTE OF PLANT BIOLOGY AND BIOTECHNOLOGY
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003186/