PXD053437-1

PXD053437 is an original dataset announced via ProteomeXchange.

Title	METTL9 sustains vertebrate neural development primarily via non-catalytic functions
Description	METTL9 is an enzyme catalysing histidine N1-methylation (1-MH). Given the high levels of this methyltransferase during vertebrate nervous system development, we dissected the roles of Mettl9 during this relevant physiological process. We generated three mouse embryonic stem cell lines featuring a complete Mettl9 knock-out, an inducible degron system, and the endogenous expression of a catalytically inactive protein. Additionally, we employed Xenopus laevis to down-regulate Mettl9 during neural development in vivo. Our multi-omic data indicate that METTL9 exerts a conserved role in sustaining vertebrate neurogenesis. Surprisingly, both perturbation of METTL9 catalytic activity and depletion of METTL9 protein levels without affecting global 1-MH levels show milder neural commitment defects, suggesting a role for both METTL9 catalytic and non-enzymatic activities. These converge towards the secretory pathway, where METTL9 co-localises with the Golgi apparatus and interacts with key regulators of cellular transport, endocytosis and Golgi integrity.
HostingRepository	PRIDE
AnnounceDate	2025-07-29
AnnouncementXML	Submission_2025-07-29_07:21:46.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	alessandro cuomo
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-26 12:19:19	ID requested
⏵ 1	2025-07-29 07:21:47	announced

10.1038/S41467-025-62414-5;

submitter keyword: Mouse, METTL9, Proteome, Golgi integrity

Luca Pandolfini
contact affiliation	Italian Institute of Technology (IIT), Center for Human Technologies, Via Enrico Melen 83, 16152 Genoa, IT
contact email	luca.pandolfini@iit.it
lab head
alessandro cuomo
contact affiliation	IEO
contact email	alessandro.cuomo@ieo.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD053437

PRIDE project URI

• PRIDE
  1. PXD053437
     1. Label: PRIDE project
     2. Name: METTL9 sustains vertebrate neural development primarily via non-catalytic functions