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PXD053391-1

PXD053391 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA classic famous prescription alleviates inflammation in CUMS model mice via modulating MYDGF/MAP4K4/NF-κB signaling pathway, verified through UPLC-HRMS and proteomics analysis
DescriptionMajor depressive disorder (MDD) has caused a heavy burden on society and affected over 350 million patients worldwide currently. However, there have been 30-50% MDD patients with non-response to antidepressants. Chronic unpredictable mild stress (CUMS) has been considered as a critical trigger factor for MDD, the pathological mechanism of which is strongly associated with stimulating inflammatory responses. Xiaoyaosan (XYS), as a classic famous prescription, has been proven effective for alleviating CUMS induced injury. Purpose: This study aimed to clarify the potential anti-inflammatory mechanisms of XYS treating CUMS model mice. Study design and methods: The therapeutic effects of XYS on the behavior and inflammatory indicators of CUMS model mice were determined. Ultra high performance liquid chromatography - High resolution mass spectrometry (UPLC-HRMS) was applied identify the compounds of XYS and proteomics analysis was utilized to explore the potential mechanism. Additionally, molecular docking and further in vivo experiments were conducted to verify the results of proteomics analysis. Results: The in vivo experiments confirmed XYS not only could significantly improve sucrose preference percentage and behavioral indicators of open field test, but also could decrease the serum levels of inflammatory indexes (CORT, hs-CRP, NFκB, FIB and Lp-PLA2) and inhibit the protein expressions of IL-6 and TNF-α in brain of CUMS model mice. Proteomics analysis presented that MAPK signaling pathway might be the important anti-inflammatory mechanism of XYS alleviating CUMS induced injury. UPLC-HRMS identify the compounds of XYS, which were taken into molecular docking to analysis the interaction between XYS and MYDGF, PKC, MAP4K4, P-p65, p65, P-IKBα, and IKBα. It was validated that XYS could exert regulatory effects on the MYDGF/MAP4K4/NF-κB signaling pathway in CUMS model mice. Conclusion: In conclusion, this study first demonstrated that XYS could alleviate inflammation in CUMS model mice via modulating MYDGF/MAP4K4/NF-κB signaling pathway.
HostingRepositoryiProX
AnnounceDate2024-06-25
AnnouncementXMLSubmission_2024-06-25_03:08:51.890.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuolan Huang
SpeciesList scientific name: Mus sp.; NCBI TaxID: 10095;
ModificationListNo PTMs are included in the dataset
InstrumentProgenesis LC-MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-25 03:07:37ID requested
12024-06-25 03:08:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Herbal medicine, Chronic unpredictable mild stress, Major depressive disorder, Proteomics analysis, Ultra high performance liquid chromatography - High resolution mass spectrometry
Contact List
Xifei Yang
contact affiliationShenzhen Key Laboratory of Modern Toxicology
contact emailxifeiyang@gmail.com
lab head
Ruolan Huang
contact affiliationShenzhen University General Hospital
contact emailruolan_huang@szu.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI