PXD053390-1

PXD053390 is an original dataset announced via ProteomeXchange.

Title	Tudor-based Proteomic Strategy Pan-specifically Enriches and Identifies Protein Arginine Methylation
Description	In current study, we developed a molecular affinity strategy based on the Tudor domain of SMN, a naturally occurring methylarginine reader protein, for comprehensive proteomic profiling of cellular arginine methylation. We demonstrated that the Tudor domain-based approach exhibits broad specificity for proteins harboring mono- or di-methylated arginines, encompassing both RGG/RG-rich and non-RG motifs, facilitating the discovery of novel methylation sites. Using this strategy, we identified asymmetric dimethylarginine (aDMA) at the N-terminal of eIF3D, an essential component of the eukaryotic translation initiation complex. Biochemical analysis revealed that aDMA modification at R99 of eIF3D plays a regulatory role in protein translation initiation. Our findings establish a generally applicable approach for proteomic profiling of arginine methylation and unveil its novel regulatory role for this modification in eukaryotic protein translation.
HostingRepository	PRIDE
AnnounceDate	2025-12-15
AnnouncementXML	Submission_2025-12-14_16:18:46.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	凌子 卢
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-25 02:49:39	ID requested
⏵ 1	2025-12-14 16:18:47	announced

Lu L, Li T, Zhang R, Wang Y, Ye X, Luo Y, Sun L, Qi L, Ye Z, Mao Y, Yuan Y, Tudor-based proteomic strategy pan-specifically enriches and identifies protein arginine methylation. EMBO Rep, 26(23):5649-5672(2025) [pubmed]

10.1038/s44319-025-00599-y;

submitter keyword: proteomics,arginine methylation, molecular affinity-enrichment method, eIF3D

Yanqiu Yuan
contact affiliation	SUN YAT-SEN UNIVERSITY
contact email	yuanyq8@mail.sysu.edu.cn
lab head
凌子 卢
contact affiliation	SUN YAT-SEN UNIVERSITY
contact email	lulz@mail2.sysu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053390
     1. Label: PRIDE project
     2. Name: Tudor-based Proteomic Strategy Pan-specifically Enriches and Identifies Protein Arginine Methylation