⮝ Full datasets listing
PXD053369-1
PXD053369 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative analysis of non histone lysine methylation sites and lysine demethylases in breast cancer cell lines |
| Description | Tandem Mass Tag labeling (TMT labels) based mass spectrometry was used to quantify lysine methylation from four different cell lines (n=4 replicates): MCF7 (ER positive breast cancer), MDAMB231 (TNBC), HCC1806 (TNBC), and MCF10A (non-cancerous), sans antibody enrichment. To boost the detection of lysine demethylases, we generated a GFP-KDM Trigger Channel. Briefly, 27 GFP-KDMs were overexpressed and immunoprecipitated from HEK293T cells. The immunoprecipitated proteins were subsequently trypsin digested, TMT labeled, and added to the Breast Cancer 16-plex. All runs were conducted on the Orbitrap Exploris and processed using Proteome Discoverer. Database searching was conducted using Sequest HTS using the Uniprot-reviewed human proteome. Downstream data and bioinformatic analysis was conducted using R. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-23 |
| AnnouncementXML | Submission_2026-01-23_10:27:08.771.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Christine Berryhill |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Dimethyl; monomethylated L-lysine; Trimethyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-24 11:58:39 | ID requested | |
| ⏵ 1 | 2026-01-23 10:27:09 | announced |
Publication List
| Berryhill CA, Evans TN, Doud EH, Smith-Kinnaman WR, Hanquier JN, Mosley AL, Cornett EM, Quantitative analysis of non-histone lysine methylation sites and lysine demethylases in breast cancer cell lines. bioRxiv, ():(2024) [pubmed] |
Keyword List
| submitter keyword: lysine methylation, Isobaric Trigger Channel, lysine demethylases, breast cancer |
Contact List
| Evan Cornett | |
|---|---|
| contact affiliation | Indiana University School of Medicine |
| contact email | evcorn@iu.edu |
| lab head | |
| Christine Berryhill | |
| contact affiliation | Indiana University School of Medicine |
| contact email | causherm@iu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095132/ |
