⮝ Full datasets listing
PXD053368-1
PXD053368 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Co-translational profiling in the cardiac endothelium in response to LPS-induced inflammation in female mice in vivo: a proof-of-concept approach |
| Description | Producing functional proteins involves multiple steps during mRNA translation on the ribosomes. However, co-translational regulatory mechanisms remain poorly characterized in intact mammalian systems. As a proof-of-concept, we developed a multi-omics approach to investigate endothelial-specific, co-translational regulation by modifying the translating ribosome affinity purification (TRAP) in vivo. We simultaneously co-immunoprecipitated (IP) polysome-associated mRNAs and proteins from the hearts of hemagglutinin-tagged ribosomal protein L22 mice (RiboTag) crossed with inducible endothelial-specific Cdh5CreERT2 mice (RiboTagEC). To perturb endothelial function, female mice were injected with E. coli lipopolysaccharide (LPS) (6 mg/Kg, i.p., 12 h). Hearts were homogenized, with ~?10% used for input RNA-Seq and proteomics controls, and the remainder for IP of ribosome-bound polyadenylated mRNA and proteins. Endothelial cell transcripts (pecam1, cdh5) were enriched?>?5-fold, while markers characteristic of other cell types were significantly depleted (?1250 overlapping terms) to identify pathways associated with concordant and discordant co-translational regulation. LPS was identified as the upstream regulator of the co-translational dataset that was concordantly regulated. Upregulated mRNAs but not proteins related to glycolysis were discordantly regulated. These findings validate our proof-of-concept multi-omics approach as a predictive platform for identifying disease-relevant pathways regulated at the co-translational level in vivo. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-19 |
| AnnouncementXML | Submission_2026-06-19_04:46:19.184.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Chad M. Warren |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Carbamyl; Carbamidomethyl |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-24 11:47:11 | ID requested | |
| ⏵ 1 | 2026-06-19 04:46:20 | announced |
Publication List
| Warren CM, Swaminathan B, Langa P, Villa SR, Thompson WC, Chrzanowska M, Kitajewski JK, Solaro RJ, Wolska BM, Goldspink PH, Co-translational profiling in the cardiac endothelium in response to LPS-induced inflammation in female mice in vivo: a proof-of-concept approach. Mol Cell Biochem, 481(6):2381-2398(2026) [pubmed] |
Keyword List
| submitter keyword: heart, translatome, Endothelium, Lipopolysaccharide, Ribosome |
Contact List
| Paul H. Goldspink | |
|---|---|
| contact affiliation | University of Illinois Chicago |
| contact email | pgolds@uic.edu |
| lab head | |
| Chad M. Warren | |
| contact affiliation | University of Illinois-Chicago |
| contact email | cmwarren@uic.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095131/ |
