PXD053361-1

PXD053361 is an original dataset announced via ProteomeXchange.

Title	ER-phagy regulates ER dynamics during myogenesis
Description	Endoplasmic reticulum (ER) plasticity and ER-phagy are intertwined processes essential for maintaining ER dynamics. We investigated the interplay between two isoforms of the ER-phagy receptor FAM134B in regulating ER remodeling in differentiating myoblasts. During myogenesis, the canonical FAM134B1 is degraded, while its isoform FAM134B2 is transcriptionally upregulated. The switch, favoring FAM134B2, indicates its significance as a regulator of ER morphology during myogenesis. FAM134B2 partial reticulon homology domain, with its rigid conformational characteristics, enables an efficient ER reshaping. FAM134B2 action increases in the active phase of differentiation leading to ER restructuring via ER-phagy, which then reverts to physiological levels when myotubes are mature and the ER reorganized. Knocking out both FAM134B isoforms in myotubes results in aberrant proteome landscape and the formation of dilated ER structures, both of which are rescued by FAM134B2 re-expression. Our results underscore how the fine tuning of FAM134B isoforms and ER-phagy orchestrate the ER dynamics during myogenesis providing insights into the molecular mechanisms governing ER homeostasis in muscle cells.
HostingRepository	PRIDE
AnnounceDate	2024-12-27
AnnouncementXML	Submission_2024-12-27_15:01:49.986.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paolo Grumati
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-24 08:59:26	ID requested
⏵ 1	2024-12-27 15:01:50	announced

10.1038/S44318-024-00356-2;

submitter keyword: FAM134B, Autophagy, ERphagy

Paolo Grumati
contact affiliation	TIGEM
contact email	p.grumati@tigem.it
lab head
Paolo Grumati
contact affiliation	TIGEM Institute
contact email	p.grumati@tigem.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053361
     1. Label: PRIDE project
     2. Name: ER-phagy regulates ER dynamics during myogenesis