PXD053345-1

PXD053345 is an original dataset announced via ProteomeXchange.

Title	Ring A cleaving beta-diketon hydrolase is a key enzyme of steroid degradation in anaerobic bacteria
Description	Bacterial degradation of the ubiquitous and persistent steroids is important for their removal from the environment, particularly for the endocrine disrupting steroid sex hormones. While aerobic bacteria use oxygenases to attack non-activated C-H/C-C bonds of the isoprenoid side chain or the steran skeleton of steroids, initial studies of steroid degradation in anaerobic bacteria suggested that water-dependent enzymes are involved in C-H hydroxylation and ring cleavage reactions. In anaerobic steroid catabolism, the hydrolytic enzymes involved in the cleavage of the steran ring system of the common intermediate androst-1,4-diene-3-one have remained unknown. Here, we have enriched a hydrolase from the cholesterol/nitrate grown Sterolibacterium denitrificans and from Escherichia coli after heterologous expression of its gene. It specifically cleaves the cyclic 1,3-diketone degradation intermediate of ring A, androsta-1,4,17-trione, to 1,17-dioxo-2,3-seco-androstan-3-oate (DSAO), a hallmark reaction of anaerobic steroid degradation. The highly conserved ring A hydrolase was identified in all known and many previously unknown steroid-degrading Proteobacteria. Using the enriched enzyme, we enzymatically produced the CoA ester of DSAO from the chemically synthesized androst-1-ene-3-one precursor, allowing the identification of subsequent metabolites involved in ring A degradation. The results obtained suggest the involvement of an additional hydrolase, an aldolase, and a -oxidation-like cascade for complete ring A degradation during which acetate and acetyl-CoA are released to form the three-ring 5,10-seco-1,2,3,4-tetranorandrosta-5,17-dione. The results identified a key enzyme of anaerobic steroid degradation that may serve as functional marker for monitoring steroid contaminant degradation at anoxic environmental sites.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_20:02:06.448.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lina Peller
SpeciesList	scientific name: Sterolibacterium denitrificans; NCBI TaxID: 157592;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-24 01:58:48	ID requested
⏵ 1	2025-05-06 20:02:07	announced

Jacoby C, Peller L, Wenzler J, Luttermann M, Seiche W, Breit B, Boll M, Ring A Cleaving Beta-Diketone Hydrolase Is a Key Enzyme of Steroid Degradation in Anaerobic Bacteria. Environ Microbiol, 27(1):e70034(2025) [pubmed]

10.1111/1462-2920.70034;

submitter keyword: Steroid degradation, LC-MS, Hydrolase,Sterolibacterium denitrificans

Matthias Boll
contact affiliation	Faculty of Biology - Microbiology, University of Freiburg,79104 Freiburg, Germany
contact email	matthias.boll@biologie.uni-freiburg.de
lab head
Lina Peller
contact affiliation	University of Freiburg
contact email	lina.peller@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053345
     1. Label: PRIDE project
     2. Name: Ring A cleaving beta-diketon hydrolase is a key enzyme of steroid degradation in anaerobic bacteria