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PXD053341-1

PXD053341 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlephoto-crosslinking mass spectrometry localises SNAPC5 and SNAPC2 in SNAPc
DescriptionRNA polymerase III (Pol III) is specialized in the transcription of short, functional RNAs, including small nuclear RNAs (snRNAs). At snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc)forming apreinitiation complex (PIC). Uniquely, SNAPc forms PICs with both Pol II and Pol IIIat their respectivepromoters. Increasingly, snRNA PIC structures arethe focus of structural characterization, however the mechanism of SNAPc cross-polymerase engagementand the role of the SNAPC2 and SNAPC5 subunits in transcription remain unclear. Here, we present CryoEM structuresoftheSNAPc-containing Pol III PIC assembled on the U6 snRNA promoterin the open and melting statesat 3.2-4.2Å resolution. Comparative structural analysis revealedunexpected differences with the corresponding yeast complex and revealedthe basis of SNAPc engagement with both Pol III and Pol IIPICs. Integrating crosslinkingmass spectrometry,we alsolocalizethe SNAPC2 and SNAPC5 subunitsin proximity to the bound promoter DNA, expanding upon existing descriptions of snRNA Pol III PIC structure.
HostingRepositoryPRIDE
AnnounceDate2025-01-06
AnnouncementXMLSubmission_2025-01-06_08:17:09.428.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD053341
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrea Graziadei
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-24 00:36:13ID requested
12025-01-06 08:17:09announced
Publication List
10.6019/PXD053341;
10.1038/S41467-024-55553-8;
Keyword List
submitter keyword: POLIII, crosslinking MS, XL-MS, RNA polymerase III, CX-MS,SNAPC, SDA
Contact List
Andrea Graziadei
contact affiliationHuman Technopole
contact emailandrea.graziadei@fht.org
lab head
Andrea Graziadei
contact affiliationHuman Technopole
contact emailandrea.graziadei@fht.org
dataset submitter
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Dataset FTP location
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