PXD053324-1
PXD053324 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS-based unlabeled quantitative proteomics |
Description | The desalted peptide solution was freeze-dried and dissolved in 0.1% formic acid for injection into a custom-made reverse-phase analytical column (15 cm long, 75 μm inner diameter). Peptides were separated using a linear gradient of mobile phase buffer B (0.1% formic acid in 98% acetonitrile) on an EASY-nLC 1000 UPLC system (Thermo Fisher Scientific). The gradient began at 6% B, increased to 23% B over 26 min, then to 35% B over 8 min, climbed to 80% B over 3 min, and was maintained at 80% B for 3 min. Eluted peptides were loaded into a nano-electrospray ionization (NSI) source and analyzed using tandem mass spectrometry on a Q Exactive™ Plus (Thermo Fisher Scientific) coupled online with the UPLC. The applied spray voltage was 2.0 kV. Full scan MS detected peptides in the m/z range of 350 to 1800 at a resolution of 70,000 in the Orbitrap. Subsequently, selected peptides were detected at a resolution of 17,500 with a normalized collision energy (NCE) of 28. MS analysis was performed in data-dependent scan mode alternating between one MS scan and 20 MS/MS scans, with a dynamic exclusion set to 15.0 s. Automatic gain control (AGC) target and fixed first mass were set to 5E4 and 100 m/z, respectively. |
HostingRepository | iProX |
AnnounceDate | 2024-06-24 |
AnnouncementXML | Submission_2024-06-23_20:06:40.245.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yanlu Ma |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-06-23 20:05:51 | ID requested | |
⏵ 1 | 2024-06-23 20:06:40 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics |
Contact List
Xin Zhang | |
---|---|
contact affiliation | Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine |
contact email | zhangxin68619@163.com |
lab head | |
Yanlu Ma | |
contact affiliation | Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine |
contact email | 2544803711@qq.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |