PXD053324-1
PXD053324 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LC-MS/MS-based unlabeled quantitative proteomics |
| Description | The desalted peptide solution was freeze-dried and dissolved in 0.1% formic acid for injection into a custom-made reverse-phase analytical column (15 cm long, 75 μm inner diameter). Peptides were separated using a linear gradient of mobile phase buffer B (0.1% formic acid in 98% acetonitrile) on an EASY-nLC 1000 UPLC system (Thermo Fisher Scientific). The gradient began at 6% B, increased to 23% B over 26 min, then to 35% B over 8 min, climbed to 80% B over 3 min, and was maintained at 80% B for 3 min. Eluted peptides were loaded into a nano-electrospray ionization (NSI) source and analyzed using tandem mass spectrometry on a Q Exactive™ Plus (Thermo Fisher Scientific) coupled online with the UPLC. The applied spray voltage was 2.0 kV. Full scan MS detected peptides in the m/z range of 350 to 1800 at a resolution of 70,000 in the Orbitrap. Subsequently, selected peptides were detected at a resolution of 17,500 with a normalized collision energy (NCE) of 28. MS analysis was performed in data-dependent scan mode alternating between one MS scan and 20 MS/MS scans, with a dynamic exclusion set to 15.0 s. Automatic gain control (AGC) target and fixed first mass were set to 5E4 and 100 m/z, respectively. |
| HostingRepository | iProX |
| AnnounceDate | 2024-06-24 |
| AnnouncementXML | Submission_2024-06-23_20:06:40.245.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yanlu Ma |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-06-23 20:05:51 | ID requested | |
| ⏵ 1 | 2024-06-23 20:06:40 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: proteomics |
Contact List
| Xin Zhang | |
|---|---|
| contact affiliation | Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine |
| contact email | zhangxin68619@163.com |
| lab head | |
| Yanlu Ma | |
| contact affiliation | Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine |
| contact email | 2544803711@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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