PXD053300-1

PXD053300 is an original dataset announced via ProteomeXchange.

Title	Histone dynamics at replication forks in embryonic stem cells
Description	We employ inducible mutants to dissect the early and late consequences of impaired histone recycling. We find rapid accumulation of H3K27me3 in response to impaired histone recycling, preceding gene expression changes. Simultaneous disruption of both leading (POLE4) and lagging strand (MCM2-2A) recycling pathways impairs transmission of parental histones to new DNA, with release of parental histones to the soluble pool. This loss of histone inheritance alters gene expression in embryonic stem cells, challenges differentiation programs and compromises cell viability. Our findings demonstrate the importance of efficient transmission of histone-based information during DNA replication for maintaining chromatin landscapes, differentiation potential, and cellular viability.
HostingRepository	PRIDE
AnnounceDate	2025-01-12
AnnouncementXML	Submission_2025-01-12_06:49:59.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Moritz Voelker-Albert
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monomethylated residue; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-22 01:52:25	ID requested
⏵ 1	2025-01-12 06:50:00	announced

Dataset with its publication pending

submitter keyword: Parental histones, POLE4, MCM2, ESCs

Anja Groth
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen 2200, Denmark
contact email	anja.groth@cpr.ku.dk
lab head
Moritz Voelker-Albert
contact affiliation	MOLEQLAR Analytics GmbH
contact email	moritz@moleqlar.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053300
     1. Label: PRIDE project
     2. Name: Histone dynamics at replication forks in embryonic stem cells