PXD053286 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Functional surface expression of immunoglobulin cleavage systems in a candidate Mycoplasma vaccine chassis |
Description | The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate virulence factor present in multiple pathogenic species of the Mollicutes, including the fast-growing species Mycoplasma feriruminatoris. The MIB-MIP system cleaves the heavy chain of host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work, using -omics technologies and 5’RACE, we show that the four copies of the M. feriruminatoris MIB-MIP system have different expression levels and are transcribed as operons controlled by four different promoters. Individual MIB-MIP gene pairs of M. feriruminatoris and other Mollicutes were introduced in an engineered M. feriruminatoris strain devoid of MIB-MIP genes and were tested for their functionality using newly developed oriC-based plasmids. The two proteins are functionally expressed at the surface of M. feriruminatoris, which confirms the possibility to display large membrane-associated proteins in this bacterium. However, functional expression of heterologous MIB-MIP systems introduced in this engineered strain from phylogenetically distant porcine Mollicutes like Mesomycoplasma hyorhinis or Mesomycoplasma hyopneumoniae could not be achieved. Finally, since M. feriruminatoris is a candidate for biomedical applications such as drug delivery, we confirmed its safety in vivo in domestic goats, which are the closest livestock relatives to its native host the Alpine ibex. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-28 |
AnnouncementXML | Submission_2024-06-28_00:35:09.961.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manfred Heller |
SpeciesList | scientific name: Mycoplasma feriruminatoris; NCBI TaxID: 1179777; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-21 09:28:21 | ID requested | |
⏵ 1 | 2024-06-28 00:35:10 | announced | |
Publication List
Keyword List
submitter keyword: genome transplantation, immunoglobulin cleavage, Mesomycoplasma hyorhinis, oriC-plasmids,Mycoplasma feriruminatoris |
Contact List
Jörg Jores |
contact affiliation | Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland |
contact email | joerg.jores@unibe.ch |
lab head | |
Manfred Heller |
contact affiliation | Proteomics and Mass Spectrometry Core Facility, Departement for Biomedical Research, University of Berne |
contact email | pmscf.dbmr@unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD053286
- Label: PRIDE project
- Name: Functional surface expression of immunoglobulin cleavage systems in a candidate Mycoplasma vaccine chassis