PXD053276-1

PXD053276 is an original dataset announced via ProteomeXchange.

Title	Multi-omics study on the effect of moderate intensity exercise on protein of lactylation in mouse muscle tissue
Description	To explore the effects of moderate intensity exercise on protein of lactylation in mouse muscle tissue metabolism. The healthy adult mice running for 6 weeks as exercise model and sedentary mice as control were used to perform transcriptomic, proteomic, lactylation-proteomics, and metabolomics analysis. In addition, correlation analysis between transcriptome and proteome, and proteome and metabolome was conducted as well. In this study, 159 lactate sites of 78 proteins were identified to be differentically regulated by moderate intensity exercise. Enrichment analysis showed that lactated proteins Mtatp8, Atp5mg and Atp5po exhibited ATP hydrolysis activity. They are involved in biological processes such as mitochondrial transmembrane transport, and Mtatp8, Atp5mg and Atp5po participate in Oxidative phosphorylation and Thermogenesis pathways. The lactation levels of Mtatp8, Atp5mg and Atp5po proteins in exercise group were significantly decreased, while their protein levels were significantly increased. The combined analysis of proteomics and metabolomics showed that the Oxocarboxylic acid metabolism pathway and Sphingolipid signaling pathway had significant changes under the influence of moderate intensity exercise. Our results showed that moderate intensity exercise has a certain effect on the lactylation level of mice, possibly by reducing lactylation levels of Mtatp8, Atp5mg and Atp5po and increasing the expression of their protein levels, thereby regulating the Oxidative phosphorylation pathway and participating in energy metabolism. 2-Oxocarboxylic acid metabolism pathway and Sphingolipid signaling pathway need to be further explored.
HostingRepository	PRIDE
AnnounceDate	2025-02-10
AnnouncementXML	Submission_2025-02-09_18:20:51.601.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wanyu Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	lactic acid
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-21 00:23:05	ID requested
⏵ 1	2025-02-09 18:20:51	announced

10.1038/S41598-024-82150-Y;

submitter keyword: lysine malonylation；polycystic ovary syndrome；SIRT4；granulosa cells；metabolic pathway

Jiahui Chang, Wanyu Wu, Ping Qian, Zhaoxu Lu, Xuejia He, Fang Wang, Ting Zhang
contact affiliation	Peking Union Medical College
contact email	wuwanyu0474230@sina.com
lab head
Wanyu Wu
contact affiliation	Peking Union Medical College
contact email	wuwanyu0474230@sina.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053276

PRIDE project URI

• PRIDE
  1. PXD053276
     1. Label: PRIDE project
     2. Name: Multi-omics study on the effect of moderate intensity exercise on protein of lactylation in mouse muscle tissue