PXD053273 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative proteomics of cobalamin-dependent propionate production by the rumen bacterium Xylanibacter ruminicola |
| Description | Xylanibacter ruminicola is an abundant rumen bacterium that produces propionate in a cobalamin-dependent manner. However, the effect of cobalamin supplementation on the expression of propionate pathway enzymes has yet to be investigated. To assess this, comparative transcriptomics and proteomics was carried out on X. ruminicola KHP1 cultures grown in defined media in the presence and absence of cobalamin. Cobalamin supplementation did not affect expression of most propionate pathway proteins, with the only differentially abundant pathway enzyme being the cobalamin-dependent methylmalonyl-CoA mutase, which showed a minor upregulation in the presence of cobalamin. Our results demonstrate expression of the propionate pathway regardless of cobalamin availability, suggesting that cobalamin-dependent induction of propionate production by X. ruminicola is controlled post-translationally. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_07:01:37.094.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sam Mahoney-Kurpe |
| SpeciesList | scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Xylanibacter ruminicola; NCBI TaxID: 839; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-06-20 23:58:14 | ID requested | |
| 1 | 2024-10-15 01:50:07 | announced | |
| ⏵ 2 | 2024-10-22 07:01:37 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: propionate,Rumen bacteria, Xylanibacter ruminicola |
Contact List
| Dr Christina D. Moon |
|---|
| contact affiliation | AgResearch Ltd, Grasslands Research Centre, Palmerston North, New Zealand. |
| contact email | christina.moon@agresearch.co.nz |
| lab head | |
| Sam Mahoney-Kurpe |
|---|
| contact affiliation | AgResearch Ltd, Grasslands Research Centre |
| contact email | sam.mahoneykurpe@agresearch.co.nz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053273
- Label: PRIDE project
- Name: Comparative proteomics of cobalamin-dependent propionate production by the rumen bacterium Xylanibacter ruminicola
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