PXD053204-1

PXD053204 is an original dataset announced via ProteomeXchange.

Title	Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells.
Description	Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbe-derived RNAs carried by outer membrane vesicles (OMVs) to host immune responses remain unclear. This study investigated the role of OMVs and OMV-associated small RNA (sRNA) species from pathogenic and commensal Bacteroides fragilis (ETBF and NTBF respectively) in eliciting different immune responses from intestinal epithelial cells. To distinguish the differences in the sRNA profiles of the two strains and their OMVs, RNA-seq, qRT-PCR, and northern blotting were conducted to identify enrichment of discrete sRNA species in OMVs, which were also differentially expressed between the two strains. To understand the effects of OMVs on pattern recognition receptors, Reporter cells of Toll-like receptor (TLR) activation were treated with OMVs, demonstrating activation of TLRs 2, 3, and 7. Treatment of Caco-2 and HT29-MTX cells with OMVs demonstrated increased expression of IL-8. Surprisingly, we discovered that degradation of RNase-accessible RNAs within ETBF OMVs, but not NTBF OMVs, resulted in vesicles with enhanced capacity to stimulate IL-8 expression, indicating that these extravesicular RNAs exert an immunosuppressive effect. This suggests a dual role for OMV-associated RNAs in modulating host immune responses, with implications for both bacterial pathogenesis and therapeutic applications.
HostingRepository	MassIVE
AnnounceDate	2024-11-23
AnnouncementXML	Submission_2024-11-23_11:34:17.410.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Conforti
SpeciesList	scientific name: Bacteroides fragilis; NCBI TaxID: 817;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	Synapt G2-S HDMS; nanoACQUITY UPLC

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-18 07:48:55	ID requested
⏵ 1	2024-11-23 11:34:17	announced

no publication

submitter keyword: Bacteroides Fragilis, ETBF, NTBF

Elyssia Gallagher
contact affiliation	Baylor University
contact email	elyssia_gallagher@baylor.edu
lab head
Jessica Conforti
contact affiliation	Baylor University
contact email	jess_conforti1@baylor.edu
dataset submitter

MassIVE dataset URI

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