PXD053198 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Trinucleotide cap analogs with tetraphosphate modifications: interaction partners in HEK293F cell lysate |
Description | This dataset was acquired to identify human proteins binding to tetraphosphate mRNA cap analogs. Affinity resins were derivatized with tetraphosphate mRNA cap analogs or a trinucleotide cap control and affinity purification performed in HEK293F cell extracts. The pulled-down proteins were eluted using soluble cap analogs, subjected to digestion with trypsin, TMT labelling, and nLC-MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-02 |
AnnouncementXML | Submission_2024-10-02_04:14:15.053.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Remigiusz Serwa |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-18 05:20:35 | ID requested | |
⏵ 1 | 2024-10-02 04:14:15 | announced | |
2 | 2024-10-22 07:00:24 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: tetraphosphate, affinity purification, HEK293F, 5′ cap,mRNA |
Contact List
Jacek Jemielity |
contact affiliation | Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland |
contact email | j.jemielity@cent.uw.edu.pl |
lab head | |
Remigiusz Serwa |
contact affiliation | The International Institute of Molecular Mechanisms and Machines, Polish Academy of Sciences |
contact email | r.serwa@imol.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053198
- Label: PRIDE project
- Name: Trinucleotide cap analogs with tetraphosphate modifications: interaction partners in HEK293F cell lysate