PXD053148-1

PXD053148 is an original dataset announced via ProteomeXchange.

Title	Label-Free Quantification Method for Assessing Sex from Modern and Ancient Bovine Tooth Enamel PART II
Description	Identification of the sex of fossil and archaeological animal remains offers many insights into their demography, mortality profiles and domestication pathways. However, due to manifold factors, sex determination of fossils is often impossible. To overcome this, we have developed an innovative protocol to determine animal’s sex applying label-free quantification (LFQ) of two unique AmelY peptides ‘LRYPYP’ (AmelY;[M+2]2+ 404.7212 m/z) and ‘LRYPYPSY’ (AmelY;[M+2]2+ 529.7689 m/z) that are solely present in males that permitted determination of the animal’s sex. We examine the application of this method to eight (8) modern cattle (Bos taurus) and (12) twelve fossil (Bos) samples from a Neolithic sites of Beisamoun in Israel (8th–7th millennium BC). Our methodology correctly determinate sex in all modern and ancient samples.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:10:18.709.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053148
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paula Kotli
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-16 09:18:02	ID requested
⏵ 1	2024-10-17 05:10:19	announced

10.6019/PXD053148;

10.1038/s41598-024-68603-4;

Kotli P, Morgenstern D, Bocquentin F, Khalaily H, Horwitz LK, Boaretto E, A label-free quantification method for assessing sex from modern and ancient bovine tooth enamel. Sci Rep, 14(1):18195(2024) [pubmed]

submitter keyword: Bos taurus,Amels, sex determination

Elisabetta Boaretto
contact affiliation	Weizmann Institute of Science Archeological Science
contact email	elisabetta.boaretto@weizmann.ac.il
lab head
Paula Kotli
contact affiliation	Weizmann Institute of Science
contact email	Paula.kotli@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053148

PRIDE project URI

• PRIDE
  1. PXD053148
     1. Label: PRIDE project
     2. Name: Label-Free Quantification Method for Assessing Sex from Modern and Ancient Bovine Tooth Enamel PART II