PXD053131-1

PXD053131 is an original dataset announced via ProteomeXchange.

Title	Modification of maize peroxidases’ activity and proteome of roots and leaves in the presence of plant oligosaccharides and cadmium
Description	We examined the effect of galactoglucomannan oligosaccharides (GGMOs) and/or Cd on the activity of peroxidases and the proteome of maize (Zea mays L.) roots and leaves. Our previous work confirmed that the application of GGMOs ameliorates symptoms of Cd stress in plants. Here, the plants were cultivated in hydroponics for 7 days, and the protein levels and peroxidase activity were estimated in intracellular, basic cell wall, and acidic cell wall protein fractions. The activity of peroxidases varied between the plant organs, as well as among the fractions and treatments. The application of GGMOs on plants suffering from Cd stress did not significantly influence the levels of peroxidases but modulated their activity, which implies posttranslational regulation. Next, we focused on the characterisation of proteins with significant changes in their levels in the GGMOs + Cd treatment. The changes in the level of various proteins (e.g., related to the defence reactions, cell wall structure/metabolism, and activation of plant hormones) caused by GGMOs and Cd presence suggested possible mechanisms of action of these oligosaccharides, which improve vitality of maize seedlings exposed to Cd.
HostingRepository	PRIDE
AnnounceDate	2025-01-14
AnnouncementXML	Submission_2025-01-14_06:34:50.368.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053131
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 10:24:36	ID requested
⏵ 1	2025-01-14 06:34:50	announced

10.6019/PXD053131;

10.1016/J.ECOENV.2025.117732;

submitter keyword: Cadmium, Proteome, Cell wall, Peroxidase, Oxidative stress

Peter Baráth
contact affiliation	Department of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
lab head
Maksym Danchenko
contact affiliation	Plant Science and Biodiversity Center SAS
contact email	maxymdan@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053131
     1. Label: PRIDE project
     2. Name: Modification of maize peroxidases’ activity and proteome of roots and leaves in the presence of plant oligosaccharides and cadmium