PXD053130-1

PXD053130 is an original dataset announced via ProteomeXchange.

Title	Selectivity profiling of inhibitor treated human cells via full proteome profiling
Description	Three selective hit compounds from an arrayed kinome abundance screen (98 kinases vs 1570 kinase inhibitors) were subjected to full proteome profiling by TMTpro 18-plex to assess their degradation selectivity in K562 cells. Src inhibitor 3 showed the most significant downregulation of the target kinase LYN. TAK285 did not significantly affect any kinase. Finally, CSK and RIPK2 (drug screen targets) were identified as the only two kinase targets downregulated by RIPK-IN-4. The compounds were further assessed for their detailed mode of action as reported with orthogonal assays such as FACS-based CRISPR/Cas9 screens and BioID.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_16:36:47.252.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053130
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fabian Frommelt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 09:48:59	ID requested
⏵ 1	2025-06-08 16:36:48	announced

Frommelt F, Ladurner R, Goldmann U, Wolf G, Ingles-Prieto A, Lineiro-Retes E, Gelov, á Z, Hopp AK, Christodoulaki E, Teoh ST, Leippe P, Santini BL, Rebsamen M, Lindinger S, Serrano I, Onstein S, Klimek C, Barbosa B, Pantielieieva A, Dvorak V, Hannich TJ, Schoenbett J, Sansig G, Mocking TAM, Ooms JF, IJzerman AP, Heitman LH, Sykacek P, Reinhardt J, M, ü, ller AC, Wiedmer T, Superti-Furga G, The solute carrier superfamily interactome. Mol Syst Biol, 21(6):632-675(2025) [pubmed]

10.1038/s44320-025-00109-1;

10.6019/PXD053130;

submitter keyword: K562, kinase inhibitors, monovalent degrader,TMT

Georg E. Winter
contact affiliation	Winter Lab, CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria (lab head)
contact email	gwinter@cemm.oeaw.ac.at
lab head
Fabian Frommelt
contact affiliation	CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
contact email	fabian.frommelt@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD053130

PRIDE project URI

• PRIDE
  1. PXD053130
     1. Label: PRIDE project
     2. Name: Selectivity profiling of inhibitor treated human cells via full proteome profiling