PXD053128-1

PXD053128 is an original dataset announced via ProteomeXchange.

Title	The immune factors involved in the rapid clearance of bacteria from the midgut of the tick Ixodes ricinus
Description	Ticks are obligate hematophagous arthropods that transmit a wide range of pathogens to humans as well as wild and domestic animals. They also harbor a non-pathogenic microbiota, although our previous study has shown that the diverse bacterial microbiome in the midgut of Ixodes ricinus is quantitatively poor and lacks a core microbe. In artificial infections by capillary feeding of ticks with two model bacteria (Gram-positive Micrococcus luteus and Gram-negative Pantoea sp.), rapid clearance of these microbes from the midgut was observed, indicating the presence of active immune mechanisms in this organ. In the current study, RNA-seq analysis was performed on the midgut of I. ricinus females inoculated with either M. luteus or Pantoea sp. or with sterile water as a control. While no immune-related transcripts were upregulated by microbial inoculation compared to the sterile control, capillary feeding itself triggered dramatic transcriptional changes in the tick midgut. Manual curation of the transcriptome from the midgut of unfed I. ricinus females, complemented by proteomic analysis, revealed the presence of several constitutively expressed putative antimicrobial peptides (AMPs) that are independent of microbial stimulation and are referred to here as ‘guard’ AMPs. These included two types of midgut-specific defensins, two different domesticated amidase effector 2 (Dae2), microplusin/ricinusin-related molecules, two lysozymes and two gamma interferon-inducible lysosomal thiol reductases (GILTs). The in vitro antimicrobial activity assays of two synthetic mature defensins, defensin 1 and defensin 8, confirmed their specificity against Gram-positive bacteria showing exceptional potency to inhibit the growth of M. luteus at nanomolar concentrations. The antimicrobial activity of midgut defensins is likely part of a multicomponent system responsible for the rapid clearance of bacteria in the tick midgut. Further studies are needed to evaluate the role of other identified ‘guard‘ AMPs in controlling microorganisms entering the tick midgut.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_05:18:22.155.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Filip Dyčka
SpeciesList	scientific name: Ixodes ricinus; NCBI TaxID: 34613;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 09:02:02	ID requested
⏵ 1	2024-10-17 05:18:23	announced

Guizzo MG, Frantov, á H, Lu S, Kozelkov, á T, Č, í, halov, á K, Dy, č, ka F, Hrbatov, á A, Tonk-R, ü, gen M, Perner J, Ribeiro JM, Foga, ç, a AC, Zurek L, Kop, á, č, ek P, . Front Cell Infect Microbiol, 14():1450353(2024) [pubmed]

10.3389/fcimb.2024.1450353;

submitter keyword: midgut microbiome, defensin, immune system, antimicrobial peptide, Micrococcus luteus,Tick, Ixodes

Petr Kopáček
contact affiliation	Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branisovska 1160/31, 37005 Ceske Budejovice, Czech Republic
contact email	kopajz@paru.cas.cz
lab head
Filip Dyčka
contact affiliation	University of South Bohemia in Ceske Budejovice Faculty of Science Branisovska 1760 Ceske Budejovice
contact email	dycka@mail.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053128

PRIDE project URI

• PRIDE
  1. PXD053128
     1. Label: PRIDE project
     2. Name: The immune factors involved in the rapid clearance of bacteria from the midgut of the tick Ixodes ricinus