PXD053115-1

PXD053115 is an original dataset announced via ProteomeXchange.

Title	Glutamine missense suppression transfer RNAs inhibit polyglutamine aggregation
Description	Mass spectrometry confirmed Ala mis-incorporation in polyQ, demonstrating misincorporation at each position in polyQ at a level of ~10% Ala at Gln codons. Cells expressing the missense suppressor tRNAs showed no growth defect or significant changes in cytotoxicity. Our findings demonstrate that tRNA-dependent missense suppression of glutamine codons is well-tolerated in cells has the potential to modify and significantly reduce protein aggregation that causes HD.
HostingRepository	PRIDE
AnnounceDate	2025-01-15
AnnouncementXML	Submission_2025-01-15_05:47:46.488.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053115
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kyle Hoffman
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-14 04:41:10	ID requested
⏵ 1	2025-01-15 05:47:46	announced

10.1016/J.OMTN.2024.102442;

10.6019/PXD053115;

submitter keyword: transfer RNA, mistranslation,Huntington's disease, polyQ

Patrick O'Donoghue
contact affiliation	Department of Biochemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada. Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5C1, Canada
contact email	patrick.odonoghue@uwo.ca
lab head
Kyle Hoffman
contact affiliation	Bioinformatics Solutions Inc.
contact email	khoffman@bioinfor.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD053115

PRIDE project URI

• PRIDE
  1. PXD053115
     1. Label: PRIDE project
     2. Name: Glutamine missense suppression transfer RNAs inhibit polyglutamine aggregation