PXD053100-2

PXD053100 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing
Description	We developed an anti-dsRNA antibody co-immunoprecipitation coupled with quantitative mass spectrometry to comprehensively identify RBPs bound to cellular dsRNAs. To minimize false positives, we conducted a quantitative mass spectrometry analysis under two additional conditions: first, by treating the samples with RNase T1 to degrade single-stranded RNAs, and second, by performing a pull-down using synthetic double-stranded RNA, poly(I:C). The interactomes through these three methods provide an unbiased and thorough characterization of potential dsRBPs bound to cellular dsRNAs.
HostingRepository	PRIDE
AnnounceDate	2025-02-26
AnnouncementXML	Submission_2025-02-26_06:33:25.325.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053100
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jong-Seo Kim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-13 22:27:39	ID requested
1	2025-02-03 07:45:08	announced
⏵ 2	2025-02-26 06:33:25	announced	2025-02-26: Updated project metadata.

Publication List

10.6019/PXD053100;

10.6019/PXD053100;

Keyword List

ProteomeXchange project tag: Human Proteome Project
submitter keyword: dsRNA, dsRBP, interferon response, viral infection, mass spectrometry, RNA sensing, CRISPR-Cas9 screening, LC-MS/MS

ProteomeXchange project tag: Human Proteome Project

submitter keyword: dsRNA, dsRBP, interferon response, viral infection, mass spectrometry, RNA sensing, CRISPR-Cas9 screening, LC-MS/MS

Contact List

Jong-Seo Kim
contact affiliation	Assistant professor, School of Biological Sciences, Seoul National University
contact email	jongseokim@snu.ac.kr
lab head
Jong-Seo Kim
contact affiliation	School of Biological Sciences, Seoul National University
contact email	jongseokim@snu.ac.kr
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053100
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD053100

PRIDE project URI

Repository Record List

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