PXD053062-1

PXD053062 is an original dataset announced via ProteomeXchange.

Title	Developmental maturation but no sex-dependent divergence of the murine CNS myelin proteome
Description	To assess whether the CNS myelin proteome varies within the same species, we used quantitative mass spectrometry to compare myelin purified from mouse brains at three developmental timepoints (P18, P75, 6 months), brains of male and female mice, and four CNS regions (cortex, corpus callosum, optic nerve, spinal cord). We utilized a data-independent acquisition (DIA) workflow with alternating low and elevated energy (MSE) and an ion mobility-enhanced version thereof (referred to as UDMSE) to achieve both, a correct quantification of exceptionally abundant myelin proteins and a comprehensive coverage of the myelin proteome. Label-free protein quantification revealed that the myelin protein composition matures between P18 and P75, and remains largely similar up to 6 months. Proteins with decreasing abundance included cytoskeleton-associated proteins (regulating sheath growth and wrapping), whereas proteins with increasing abundance included all subunits of the septin filament (stabilizing mature myelin), and potentially protective proteins. Among the latter was quinoid dihydropteridine reductase (QDPR), which emerges as a highly specific marker for mature oligodendrocytes and myelin. Conversely, female and male mice display essentially similar myelin proteomes and the myelin-enriched fractions from the four CNS regions mainly differ by the abundance of HCN2-channels in spinal cord myelin as a requirement for particularly long sheaths, apart from the extent of contaminating mitochondrial and synaptic proteins.
HostingRepository	PRIDE
AnnounceDate	2024-08-27
AnnouncementXML	Submission_2024-08-27_14:27:34.445.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 23:48:50	ID requested
⏵ 1	2024-08-27 14:27:34	announced
2	2024-10-22 06:56:47	announced	2024-10-22: Updated project metadata.

10.1002/GLIA.24614;

submitter keyword: axon/glia-interaction, label-free protein quantification, white matter, mass spectrometry, myelin maturation, oligodendrocyte,Central nervous system (CNS), myelin proteome

Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD053062

PRIDE project URI

• PRIDE
  1. PXD053062
     1. Label: PRIDE project
     2. Name: Developmental maturation but no sex-dependent divergence of the murine CNS myelin proteome