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PXD053060-1

PXD053060 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA histone deacetylase confers plant tolerance to heat stress by controlling protein lysine deacetylation and stress granule formation in rice
DescriptionElevated temperatures due to global warming seriously threaten crops production. Understanding molecular mechanisms of cellular responses to heat stress will help to improve crop tolerance to high temperature and yield. In this work, we show that deacetylation of non-histone proteins mediated by the rice cytoplasmic histone deacetylase HDA714 is required for plant tolerance to heat stress. HDA714 expression and protein accumulation are induced by heat stress. HDA714 loss-of-function affects specifically plant response to heat (42°C) while its over-expression enhances plant tolerance to the stress. Interestingly, heat stress led to decreases of overall protein lysine acetylation in rice plants, which depends on HDA714 function. HDA714-mediated deacetylation of metabolic enzymes stimulates glycolysis under heat stress. In addition, HDA714 protein is found within heat-induced stress granules (SGs), many identified rice SG proteins show lysine acetylation at normal temperature (25 °C), which is augmented in hda714 mutants. Finally, HDA714 interacts with and deacetylates several SG proteins and HDA714 loss-of-function impairs SG formation. Collectively, these results indicate that HDA714-mediated cellular protein lysine deacetylation responds to heat stress, affects metabolic activities, regulates SGs formation, and confers heat tolerance in rice plants.
HostingRepositoryPRIDE
AnnounceDate2024-09-04
AnnouncementXMLSubmission_2024-09-04_08:30:42.587.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterQiutao Xu
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationListacetylated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-06-12 21:49:58ID requested
12024-09-04 08:30:42announced
22024-10-22 06:57:41announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SG, Acetylome,Rice,Protein acetylation, Heat stress
Contact List
Zhengting Chen, Qiutao Xu, Jing Wang, Hebo Zhao, Yaping Yue, Biao Liu, Yu Zhao, Dao-Xiu Zhou
contact affiliationAffiliation of Zhengting Chen, Jing Wang, Hebo Zhao, Yaping Yue, Biao Liu, Yu Zhao, Dao-Xiu Zhou: National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China
contact emailczt@webmail.hzau.edu.cn
lab head
Qiutao Xu
contact affiliationGuangxi University
contact emailqiutaoxu@gxu.edu.cn
dataset submitter
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Dataset FTP location
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