PXD053060-1

PXD053060 is an original dataset announced via ProteomeXchange.

Title	A histone deacetylase confers plant tolerance to heat stress by controlling protein lysine deacetylation and stress granule formation in rice
Description	Elevated temperatures due to global warming seriously threaten crops production. Understanding molecular mechanisms of cellular responses to heat stress will help to improve crop tolerance to high temperature and yield. In this work, we show that deacetylation of non-histone proteins mediated by the rice cytoplasmic histone deacetylase HDA714 is required for plant tolerance to heat stress. HDA714 expression and protein accumulation are induced by heat stress. HDA714 loss-of-function affects specifically plant response to heat (42°C) while its over-expression enhances plant tolerance to the stress. Interestingly, heat stress led to decreases of overall protein lysine acetylation in rice plants, which depends on HDA714 function. HDA714-mediated deacetylation of metabolic enzymes stimulates glycolysis under heat stress. In addition, HDA714 protein is found within heat-induced stress granules (SGs), many identified rice SG proteins show lysine acetylation at normal temperature (25 °C), which is augmented in hda714 mutants. Finally, HDA714 interacts with and deacetylates several SG proteins and HDA714 loss-of-function impairs SG formation. Collectively, these results indicate that HDA714-mediated cellular protein lysine deacetylation responds to heat stress, affects metabolic activities, regulates SGs formation, and confers heat tolerance in rice plants.
HostingRepository	PRIDE
AnnounceDate	2024-09-04
AnnouncementXML	Submission_2024-09-04_08:30:42.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiutao Xu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 21:49:58	ID requested
⏵ 1	2024-09-04 08:30:42	announced
2	2024-10-22 06:57:41	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: SG, Acetylome,Rice，Protein acetylation, Heat stress

Zhengting Chen, Qiutao Xu, Jing Wang, Hebo Zhao, Yaping Yue, Biao Liu, Yu Zhao, Dao-Xiu Zhou
contact affiliation	Affiliation of Zhengting Chen, Jing Wang, Hebo Zhao, Yaping Yue, Biao Liu, Yu Zhao, Dao-Xiu Zhou: National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China
contact email	czt@webmail.hzau.edu.cn
lab head
Qiutao Xu
contact affiliation	Guangxi University
contact email	qiutaoxu@gxu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053060
     1. Label: PRIDE project
     2. Name: A histone deacetylase confers plant tolerance to heat stress by controlling protein lysine deacetylation and stress granule formation in rice