PXD053053 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multiplexed single cell proteomics of hypoxia in HEK293 expressing PIP-FUCCI |
| Description | This study aimed to characterize temporal proteome dynamics of hypoxia using single-cell mass spectrometry-based proteomics. HEK293 cells expressing PIP-FUCCI cell cycle markers were grown in suspension culture. Cells were exposed to hypoxia and temporally sampled for single-cell sorting into 384-well plates. Samples were processed and multiplexed using TMT labeling according to the SCoPE2 workflow and analyzed using the RETICLE acquisition method. In parallel, single-cell RNA-seq data were also collected. These datasets were used together to disentangle the effects of hypoxia from cell cycle progression and to construct joint transcriptional and translational trajectories along the hypoxia response. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-16 |
| AnnouncementXML | Submission_2026-02-15_18:03:19.453.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Craig Barry |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-06-12 18:57:11 | ID requested | |
| ⏵ 1 | 2026-02-15 18:03:20 | announced | |
Publication List
| 10.1016/j.mcpro.2025.101475; |
| Barry CP, Talbo GH, Beauglehole A, Ovchinnikov D, Munro T, Mahler S, Baker K, Nielsen LK, Mercer TR, Marcellin E, Resolving Single-Cell Gene Expression by Pseudotemporal Integration of Transcriptomic and Proteomic Datasets. Mol Cell Proteomics, 25(1):101475(2026) [pubmed] |
Keyword List
| submitter keyword: single cell proteomics, hypoxia, HEK293, scMS |
Contact List
| Craig Barry |
|---|
| contact affiliation | Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, 4072 St. Lucia, Australia. |
| contact email | craig.barry@uq.net.au |
| lab head | |
| Craig Barry |
|---|
| contact affiliation | Australian Institute for Bioengineering and Nanotechnology |
| contact email | craig.barry@uq.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD053053 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053053
- Label: PRIDE project
- Name: Multiplexed single cell proteomics of hypoxia in HEK293 expressing PIP-FUCCI
