PXD053049 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway |
Description | Despite being essential for fertility, genome defence pathway genes often evolve rapidly. However, little is known about the molecular basis of this adaptation. Here, we characterize the evolution of a protein interaction network within the PIWI-interacting small RNA (piRNA) genome defence pathway in Drosophila at unprecedented scale and evolutionary resolution. We uncover pervasive rapid evolution of a protein interaction network anchored at the Heterochromatin Protein 1 (HP1) paralog Rhino. Using complementary phylogenetic analysis, high-throughput yeast-two-hybrid matrix screening, and in vivo interaction analyses in cross-species transgenic flies, we characterized at least three distinct evolutionary protein interaction trajectories across ~40 million years of Drosophila evolution. The comprehensive cross-species interaction data set covering 11 piRNA pathway proteins of five Drosophila species revealed several protein interactions that are fully conserved, indicating functional conservation despite overall rapid amino acid sequence change. Other interactions are preserved through co-evolution and were detected only between proteins in closely related and within species. We also identified sets of species-restricted protein interactions which, through rewiring of a Rhino-anchored transcription factor network, may preserve critical roles in enabling and adapting piRNA production from heterochromatic loci. In sum, our analyses dissected principles of interaction evolution in an adaptively evolving protein-protein interaction network uncovering evolutionary and functional insight into germline piRNA production across Drosophila species. Our work provides key experimental evidence in support of a fundamental model proposing that intermolecular interaction innovation is a major molecular mechanism of evolutionary adaptation in protein-coding genes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-05 |
AnnouncementXML | Submission_2024-10-05_13:59:48.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sarah Masser |
SpeciesList | scientific name: Drosophila persimilis; NCBI TaxID: 7234; scientific name: Drosophila simulans; NCBI TaxID: 7240; scientific name: Drosophila virilis; NCBI TaxID: 7244; scientific name: Drosophila erecta; NCBI TaxID: 7220; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-12 11:59:00 | ID requested | |
⏵ 1 | 2024-10-05 13:59:48 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: piRNA pathway, inter and intra lethalities,Drosophila,species-specific protein interactions,evolution |
Contact List
Ulrich Stelzl |
contact affiliation | University of Graz, Pharmaceutical Sciences, Pharmaceutical Chemistry |
contact email | ulrich.stelzl@uni-graz.at |
lab head | |
Sarah Masser |
contact affiliation | University of Graz, Institute of Pharmaceutical Sciences |
contact email | sarah.masser@uni-graz.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053049
- Label: PRIDE project
- Name: Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway