PXD053049-1

PXD053049 is an original dataset announced via ProteomeXchange.

Title	Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway
Description	Despite being essential for fertility, genome defence pathway genes often evolve rapidly. However, little is known about the molecular basis of this adaptation. Here, we characterize the evolution of a protein interaction network within the PIWI-interacting small RNA (piRNA) genome defence pathway in Drosophila at unprecedented scale and evolutionary resolution. We uncover pervasive rapid evolution of a protein interaction network anchored at the Heterochromatin Protein 1 (HP1) paralog Rhino. Using complementary phylogenetic analysis, high-throughput yeast-two-hybrid matrix screening, and in vivo interaction analyses in cross-species transgenic flies, we characterized at least three distinct evolutionary protein interaction trajectories across ~40 million years of Drosophila evolution. The comprehensive cross-species interaction data set covering 11 piRNA pathway proteins of five Drosophila species revealed several protein interactions that are fully conserved, indicating functional conservation despite overall rapid amino acid sequence change. Other interactions are preserved through co-evolution and were detected only between proteins in closely related and within species. We also identified sets of species-restricted protein interactions which, through rewiring of a Rhino-anchored transcription factor network, may preserve critical roles in enabling and adapting piRNA production from heterochromatic loci. In sum, our analyses dissected principles of interaction evolution in an adaptively evolving protein-protein interaction network uncovering evolutionary and functional insight into germline piRNA production across Drosophila species. Our work provides key experimental evidence in support of a fundamental model proposing that intermolecular interaction innovation is a major molecular mechanism of evolutionary adaptation in protein-coding genes.
HostingRepository	PRIDE
AnnounceDate	2024-10-05
AnnouncementXML	Submission_2024-10-05_13:59:48.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Masser
SpeciesList	scientific name: Drosophila persimilis; NCBI TaxID: 7234; scientific name: Drosophila simulans; NCBI TaxID: 7240; scientific name: Drosophila virilis; NCBI TaxID: 7244; scientific name: Drosophila erecta; NCBI TaxID: 7220; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 11:59:00	ID requested
⏵ 1	2024-10-05 13:59:48	announced

Dataset with its publication pending

submitter keyword: piRNA pathway, inter and intra lethalities,Drosophila,species-specific protein interactions,evolution

Ulrich Stelzl
contact affiliation	University of Graz, Pharmaceutical Sciences, Pharmaceutical Chemistry
contact email	ulrich.stelzl@uni-graz.at
lab head
Sarah Masser
contact affiliation	University of Graz, Institute of Pharmaceutical Sciences
contact email	sarah.masser@uni-graz.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053049

PRIDE project URI

• PRIDE
  1. PXD053049
     1. Label: PRIDE project
     2. Name: Recurrent innovation of protein-protein interactions in the Drosophila piRNA pathway