PXD053045-1

PXD053045 is an original dataset announced via ProteomeXchange.

Title	A microscopy-based screen identifies cellular kinases modulating mitochondrial translation
Description	Mitochondrial DNA encodes thirteen subunits of the oxidative phosphorylation (OXPHOS) system, which are synthesized inside the organelle and essential for cellular energy supply. How mitochondrial gene expression is regulated and integrated into cellular physiology is little understood. Here, we performed a high-throughput screen combining fluorescent-labelling of mitochondrial translation products with siRNA-mediated knockdown, to identify cellular kinases regulating translation. As proof of principle, the screen identified known kinases that affect mitochondrial translation, and it also revealed several kinases not yet linked to this process. Among the latter, we focused on the primarily cytosolic kinase FN3K, which localizes partially to mitochondria, to support translation. Mass spectrometric (MS) bottom-up analysis of peptide samples after FN3K-flag immunoisolation in isolated mitochondria (transfected with FN3K-flag plasmid) enabled the identification of several proteins of the mitochondrial ribosome to be interacting with FN3K. Further experiments showed that FN3K likely modulates the assembly of mitochondrial ribosomes, thereby affecting translation. Overall, our work provides a reliable approach to identify new protein functions for mitochondrial gene expression, in a high throughput manner.
HostingRepository	PRIDE
AnnounceDate	2025-01-02
AnnouncementXML	Submission_2025-01-02_02:54:15.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carina Hansohn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 08:54:05	ID requested
⏵ 1	2025-01-02 02:54:15	announced

10.1016/J.CELREP.2024.115143;

submitter keyword: mitochondrial translation, DIA, immunoisolation, cellular kinases, siRNA library, click chemistry

Henning Urlaub
contact affiliation	Max Planck Institute for Multidisciplinary Sciences, Bioanalytical Mass Spectrometry Group, Göttingen, Germany University Medical Center Göttingen, Institute of Clinical Chemistry, Bioanalytics Group, Göttingen, Germany
contact email	henning.urlaub@mpinat.mpg.de
lab head
Carina Hansohn
contact affiliation	Max Planck Institute for Multidisciplinary Sciences, Göttingen, Germany University Medical Center Goettingen, Göttingen, Germany
contact email	carina.hansohn@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD053045

PRIDE project URI

• PRIDE
  1. PXD053045
     1. Label: PRIDE project
     2. Name: A microscopy-based screen identifies cellular kinases modulating mitochondrial translation