PXD053041-1

PXD053041 is an original dataset announced via ProteomeXchange.

Title	Protein target identification of CL16 in HCT116 cells by an alkyne probe, CL16-alkyne
Description	By coupling activity-based protein profiling (ABPP) with covalent ligand screening experiment, we have identified a cysteine-reactive covalent ligand, CL16, which can bind strongly and selectively with RhoA in vitro. In this experiment, we investigated the protein targets of CL16 in colorectal cancer HCT116 cells by a molecular probe of CL16, CL16-alkyne. HCT116 cells were pretreated with solvent vehicle or CL16, followed by CL16-alkyne. The cells were lysed, and the CL16-alkyne-labeled proteins were then installed with desthiobiotin through copper-catalyzed azide-alkyne cycloaddition (CuAAC), enriched, tryptic digested and profiled by LC-MS/MS. Peptides with fold change (Control/CL16)>4 and p<0.05 (i.e. at least 75% occupancy and statistical significance) were considered as targets bound with CL16.
HostingRepository	PRIDE
AnnounceDate	2025-08-30
AnnouncementXML	Submission_2025-08-30_02:33:30.266.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clive Yik-Sham CHUNG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 07:22:06	ID requested
⏵ 1	2025-08-30 02:33:30	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS experiment on HCT116 cells probed by CL16-alkyne to investigate protein targets of CL16

Clive Yik-Sham Chung
contact affiliation	School of Biomedical Sciences and Department of Pathology, The University of Hong Kong
contact email	cyschung@hku.hk
lab head
Clive Yik-Sham CHUNG
contact affiliation	The University of Hong Kong
contact email	cyschung@hku.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053041
     1. Label: PRIDE project
     2. Name: Protein target identification of CL16 in HCT116 cells by an alkyne probe, CL16-alkyne