PXD053036-1

PXD053036 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics experiments to identify covalent modification of proteins in HCT116 cells by a novel covalent ligand, CL16
Description	By coupling activity-based protein profiling (ABPP) with covalent ligand screening experiment, we have identified a cysteine-reactive covalent ligand, CL16, which can bind strongly and selectively with RhoA in vitro. In this experiment, we performed shotgun proteomics to investigate protein targets of CL16 in colorectal cancer HCT116 cells.
HostingRepository	PRIDE
AnnounceDate	2025-08-30
AnnouncementXML	Submission_2025-08-30_01:57:27.227.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Clive Yik-Sham CHUNG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-12 05:06:24	ID requested
⏵ 1	2025-08-30 01:57:27	announced

Dataset with its publication pending

submitter keyword: Protein target identification of a cysteine-reactive covalent ligand, in colorectal cancer HCT116 cells, CL16

Clive Yik-Sham Chung
contact affiliation	School of Biomedical Sciences and Department of Pathology, The University of Hong Kong
contact email	cyschung@hku.hk
lab head
Clive Yik-Sham CHUNG
contact affiliation	The University of Hong Kong
contact email	cyschung@hku.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053036
     1. Label: PRIDE project
     2. Name: Shotgun proteomics experiments to identify covalent modification of proteins in HCT116 cells by a novel covalent ligand, CL16