PXD053015 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cellular Resiliency and Survival of a Neuro-2a Cell Line Under Extreme Stress |
Description | Stressors such as hypoxia, hypothermia, and acute toxicity often result in widespread cell death. Recently, the failure of a cryostorage dewar in our lab resulted in prolonged thawing of Neuro-2a cells, exposing them to a combination of these insults. Surprisingly, a small fraction of the cells survived the event, underwent a period of near-dormancy, and were able to fully recover to a healthy state. To investigate this unusual resiliency, we established a novel standardized model to recapitulate the event and charted the phenotypes, transcriptomes, proteomes, and mitochondrial dynamics of the surviving cells along the trajectory of recovery. We observed an enrichment in DNA-repair and chromatin modification pathways bolstered by highly elevated mitochondrial activity in the immediate aftermath of the event and through the early stages of recovery, with subsequent normalization toward that of controls as the cells completed their recovery. Our results indicate that a robust energetic state that helps sustain DNA repair and stress-responsive pathways at the earliest stages of recovery facilitate cell survival and resiliency following extreme stress. |
HostingRepository | PRIDE |
AnnounceDate | 2024-11-26 |
AnnouncementXML | Submission_2024-11-26_10:00:20.508.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stanley Stevens |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-11 10:03:53 | ID requested | |
⏵ 1 | 2024-11-26 10:00:21 | announced | |
Publication List
Hernandez-Jimenez R, Patel A, Machado-Olavarria A, Mathieu H, Wohlfahrt J, Guergues J, Stevens SM, Dharap A, Cellular resiliency and survival of Neuro-2a cells under extreme stress. Exp Cell Res, 443(1):114275(2024) [pubmed] |
10.1016/j.yexcr.2024.114275; |
Keyword List
submitter keyword: cryostorage, temperature, thawing, resiliency, stress, survival,cell, dewar |
Contact List
Dr. Stanley Stevens |
contact affiliation | Deptartment of MBS, University of South Florida |
contact email | smstevens@usf.edu |
lab head | |
Stanley Stevens |
contact affiliation | University of South Florida |
contact email | smstevens@usf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD053015 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053015
- Label: PRIDE project
- Name: Cellular Resiliency and Survival of a Neuro-2a Cell Line Under Extreme Stress