PXD053011-1

PXD053011 is an original dataset announced via ProteomeXchange.

Title	Global proteomic and phosphoproteomic analysis of WT HEC-50B and MAPK14-/- HEC-50B cells cultured as 2D monolayers and 3D spheroids.
Description	The molecular underpinnings of High Grade Endometrial Carcinoma (HGEC) metastatic growth and survival are poorly understood. Here we show that ascites-derived and primary tumor HGEC cell lines in 3D spheroid culture faithfully recapitulate key features of malignant peritoneal effusion and exhibit fundamentally distinct transcriptomic, proteomic and metabolomic landscapes when compared with conventional 2D monolayers. Using genetic screening platform we identify MAPK14 (which encodes the protein kinase p38a) as a specific requirement for HGEC in spheroid culture. MAPK14/p38a has broad roles in programing the phosphoproteome of HGEC spheroids, yet has negligible impact on monolayer cultures. Using global proteomic and phosphoproteomic analysis we identified several kinases specifically activated in 3D spheroids. The largest increases and decreases in phosphoprotein abundance caused by spheroid culture were all MAPK14-dependent. Therefore, MAPK14 plays a key role in specifying the global kinome of HGEC cells in spheroid culture.
HostingRepository	PRIDE
AnnounceDate	2025-10-07
AnnouncementXML	Submission_2025-10-07_11:38:20.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Lyons
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-11 08:26:38	ID requested
⏵ 1	2025-10-07 11:38:21	announced

10.1016/J.CELREP.2024.115104;

submitter keyword: Endometrial Cancer, p38a,MAPK14, 3D spheroids

Cyrus Vaziri
contact affiliation	Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599, USA
contact email	cyrus_vaziri@med.unc.edu
lab head
Scott Lyons
contact affiliation	UNC Chapel Hill
contact email	scott_lyons@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD053011

PRIDE project URI

• PRIDE
  1. PXD053011
     1. Label: PRIDE project
     2. Name: Global proteomic and phosphoproteomic analysis of WT HEC-50B and MAPK14-/- HEC-50B cells cultured as 2D monolayers and 3D spheroids.