PXD053005-1

PXD053005 is an original dataset announced via ProteomeXchange.

Title	Modulation of biological activities in adipose derived stem cells by histone deacetylation
Description	Epigenetic pharmaceuticals hold the promise of using cell-based regeneration to revolutionize wound healing treatment. Histone acetylation and deacetylation conducts an intriguing way that influences the fate of stem cells. In this context, we used pharmacological manipulation to coordinate histone acetylation in stem cells. When HDACs expression reduced or silenced using HDAC inhibitors, such as Suberoylanilide hydroxamic acid (SAHA), a spectacular transformation occurred at the cellular and the molecular levels. In our study, we used adipose-derived stem cells which received consecutive SAHA treatment for 72 hours. No change in the cell viability could be noticed at the studied concentrations. On the other hand, a decrease in the protein expression of histone deacetylases at 1000 nM was found. The cell proliferation marker (Ki-67) was increased after SAHA treatment compared to the vehicle as shown by immunofluorescence staining. The expression of CCND1 gene expression was upregulated, while the protein expression of the proliferating cell nuclear antigen was downregulated. Alternatively, microarray analysis revealed that CDKN1A and MDM2 genes were upregulated under the influence of SAHA. On the other hand, the mass spectrometry pathway enrichment analysis showed that cell cycle, ATP-dependent chromatin remodeling and DNA are among the regulated pathways. This study suggested that SAHA as pharmaco-epigenetic agent might alter ADSCs determination through coordinated biological processes.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_19:10:16.161.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sallam Abdallah
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-11 05:08:51	ID requested
⏵ 1	2025-05-06 19:10:17	announced

10.1038/s41598-024-84652-1;

Abdallah S, Tabebi M, Qanadilo S, Ali N, Wang J, D'Arcy P, Zhong W, Sjoberg F, Elmasry M, El-Serafi A, Modulation of biological activities in adipose derived stem cells by histone deacetylation. Sci Rep, 15(1):3629(2025) [pubmed]

submitter keyword: Histone deacetylase inhibitor, Suberoylanilide hydroxamic acid, differentiation, adipose derived stem cells, epigenetic, epigenetic modifier, Vorinostat

Sallam A bdallah
contact affiliation	PhD student, Linköping University
contact email	sallam.abdallah@liu.se
lab head
Sallam Abdallah
contact affiliation	BKV linköping University
contact email	sallam.abdallah@liu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053005

PRIDE project URI

• PRIDE
  1. PXD053005
     1. Label: PRIDE project
     2. Name: Modulation of biological activities in adipose derived stem cells by histone deacetylation