PXD052976-1

PXD052976 is an original dataset announced via ProteomeXchange.

Title	Haplotype variability in mitochondrial rRNA predisposes to metabolic syndrome
Description	Metabolic syndrome is a growing concern in developed societies and due to its polygenic nature, the genetic component is only slowly being elucidated. Common mitochondrial DNA sequence variants have been associated with symptoms of metabolic syndrome and may therefore be relevant players in the genetics of metabolic syndrome. We investigate the effect of mitochondrial sequence variation on the metabolic phenotype in conplastic rat strains with identical nuclear but unique mitochondrial genomes, challenged by high-fat diet. We find that the variation in mitochondrial rRNA sequence represents risk factor in the insulin resistance development, which is caused by diacylglycerols accumulation induced by tissue-specific reduction of the oxidative capacity. These metabolic perturbations stem from the 12S rRNA sequence variation affecting mitochondrial ribosome assembly and translation. Our work demonstrates that physiological variation in mitochondrial rRNA might represent a relevant underlying factor in the progression of metabolic syndrome.
HostingRepository	PRIDE
AnnounceDate	2024-08-13
AnnouncementXML	Submission_2024-08-13_03:26:14.278.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marek Vrbacky
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-10 14:58:52	ID requested
⏵ 1	2024-08-13 03:26:14	announced
2	2024-10-22 06:54:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: metabolic syndrome, diacylglycerols, mitochondrial haplotype, insulin signaling

Tomas Mracek
contact affiliation	Laboratory of Bioenergetics Institute of Physiology of the Czech Academy of Sciences Prague, Czech Republic
contact email	tomas.mracek@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliation	Czech Academy of Sciences
contact email	proteom.krc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052976
     1. Label: PRIDE project
     2. Name: Haplotype variability in mitochondrial rRNA predisposes to metabolic syndrome