PXD052938-1

PXD052938 is an original dataset announced via ProteomeXchange.

Title	Carotid endarterectomy tissue and tissue-derived extracellular vesicle proteomes from symptomatic and asymptomatic patients
Description	This study sought to compare how the tissue extracellular vesicle proteome of carotid atherosclerotic plaques differs between symptomatic and asymptomatic patients. Carotid arteries samples were collected from symptomatic and asymptomatic patients undergoing endarterectomies. The specimen was grossly dissected in to 1) the maximally diseased plaque zone and 2) adjacent marginal zones. Extracellular vesicles were isolated from these matched tissue regions from each specimen using collagenase digestion, size exclusion chromatography, and density gradient ultracentrifugation. Label-free proteomics was performed on both the intact tissues (N=5) and the tissue derived EVs (N=23: symptomatic patients N=13, asymptomatic patients N=10).
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_16:21:41.516.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052938
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mandy Turner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-07 14:39:26	ID requested
⏵ 1	2025-06-29 16:21:42	announced

10.6019/PXD052938;

Raju S, Turner ME, Cao C, Abdul-Samad M, Punwasi N, Blaser MC, Cahalane RME, Botts SR, Prajapati K, Patel S, Wu R, Gustafson D, Galant NJ, Fiddes L, Chemaly M, Hedin U, Matic L, Seidman MA, Subasri V, Singh SA, Aikawa E, Fish JE, Howe KL, Multiomic Landscape of Extracellular Vesicles in Human Carotid Atherosclerotic Plaque Reveals Endothelial Communication Networks. Arterioscler Thromb Vasc Biol, 45(7):1277-1305(2025) [pubmed]

10.1161/atvbaha.124.322324;

submitter keyword: Atheroslerosis, Cardiovascular, Carotid, Human, Extracellular Vesicles

Kathryn L
contact affiliation	University Health Network, Division of Vascular Surgery, Toronto General Hospital Research Institute, Division of Biomedical Research; Department of Surgery, University of Toronto; Toronto, Canada
contact email	kathryn.howe@uhn.ca
lab head
Mandy Turner
contact affiliation	Brigham and Women's Hospital, Harvard Medical School
contact email	mturner14@bwh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD052938

PRIDE project URI

• PRIDE
  1. PXD052938
     1. Label: PRIDE project
     2. Name: Carotid endarterectomy tissue and tissue-derived extracellular vesicle proteomes from symptomatic and asymptomatic patients