PXD052932-1

PXD052932 is an original dataset announced via ProteomeXchange.

Title	A Labrador PeptideAtlas - resource for proteomics research in dogs
Description	The domestic dog, Canis lupus familiaris, plays a vital role as companion or service animal, and the well-being and healthy aging of dogs is gaining importance. Proteins are key actors in cells, tissues and body fluids, and the ability to measure their composition and abundance is crucial to understand biological processes. We report a mass spectrometry-based proteomics approach analyzing canine tissue, plasma and urine to develop two comprehensive resources to advance the study of the dog proteome. Firstly, we developed a canine PeptideAtlas covering 49% of the predicted UniProt KB proteome and secondly, a spectral assay library for targeted applications by data-independent acquisition (DIA) that enables the identification and quantification of 11,792 proteins (11,564 protein groups) of the dog proteome (56%). We demonstrate the performance of the library with gradients of different length and quantify 10,140 proteins in tissue and 385 proteins in plasma.
HostingRepository	PRIDE
AnnounceDate	2025-12-18
AnnouncementXML	Submission_2025-12-18_02:28:48.506.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrike Kusebauch
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: NEWT:9615;
ModificationList	(R)-5-oxo-1; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-07 08:34:20	ID requested
⏵ 1	2025-12-18 02:28:49	announced

Dataset with its publication pending

submitter keyword: None

Robert L.
contact affiliation	Institute for Systems Biology, Seattle, WA, USA
contact email	rmoritz@systemsbiology.org
lab head
Ulrike Kusebauch
contact affiliation	Institute for Systems Biology, Seattle, WA, USA
contact email	ukusebauch@systemsbiology.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD052932

PRIDE project URI

• PRIDE
  1. PXD052932
     1. Label: PRIDE project
     2. Name: A Labrador PeptideAtlas - resource for proteomics research in dogs