PXD052922-2

PXD052922 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei
Description	KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. Using a kinetoplastid parasite Trypanosoma brucei, here we performed immunoprecipitation and mass spectrometry of GFP-tagged KKT23 N-terminal fragment (2–150) to identify co-purifying proteins, and immunoprecipitation and mass spectrometry of H2B-GFP after KKT23 depletion to examine acetylation states of histones. We also performed in vitro acetylation assays on recombinant trypanosome nucleosomes using recombinant KKT23 protein, followed by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:55:26.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bungo Akiyoshi
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-07 05:42:43	ID requested
1	2024-08-19 05:09:45	announced
⏵ 2	2024-10-22 06:55:26	announced	2024-10-22: Updated project metadata.

10.1101/2024.08.16.608276;

submitter keyword: Trypanosome kinetoplastid kinetochore KKT23 GCN5

Bungo Akiyoshi
contact affiliation	University of Edinburgh
contact email	bungo.akiyoshi@ed.ac.uk
lab head
Bungo Akiyoshi
contact affiliation	University of Edinburgh
contact email	bungo.akiyoshi@ed.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052922
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei