PXD052922 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei |
Description | KKT23 is a kinetoplastid kinetochore protein that has structural similarity to the GCN5 acetyltransferase domain. Using a kinetoplastid parasite Trypanosoma brucei, here we performed immunoprecipitation and mass spectrometry of GFP-tagged KKT23 N-terminal fragment (2–150) to identify co-purifying proteins, and immunoprecipitation and mass spectrometry of H2B-GFP after KKT23 depletion to examine acetylation states of histones. We also performed in vitro acetylation assays on recombinant trypanosome nucleosomes using recombinant KKT23 protein, followed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:55:26.438.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Bungo Akiyoshi |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-07 05:42:43 | ID requested | |
1 | 2024-08-19 05:09:45 | announced | |
⏵ 2 | 2024-10-22 06:55:26 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Trypanosome kinetoplastid kinetochore KKT23 GCN5 |
Contact List
Bungo Akiyoshi |
contact affiliation | University of Edinburgh |
contact email | bungo.akiyoshi@ed.ac.uk |
lab head | |
Bungo Akiyoshi |
contact affiliation | University of Edinburgh |
contact email | bungo.akiyoshi@ed.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD052922
- Label: PRIDE project
- Name: Mass spectrometry analysis of kinetoplastid kinetochore proteins (KKT23 acetyltransferase and its interactor KKT22) and histone acetylation levels in Trypanosoma brucei