PXD052899-1

PXD052899 is an original dataset announced via ProteomeXchange.

Title	Comparative brain proteomic analysis between sham and cerebral ischemia experimental groups
Description	In experimental animal studies, control sham groups are essential to reduce the influence of the surgical intervention on the analysis. The intraluminal filament procedure is one of the most common models of middle cerebral artery occlusion (MCAO) used in the study of brain ischemia. However, in these studies, the sham group has not usually been included in the experimental design. In this study, we aimed to evaluate the relevance of the sham group by analyzing and comparing the brain protein profile between a sham and an MCAO ischemic group. In the sham group, 98 dysregulated proteins were detected compared to the 171 in the ischemic group. Moreover, a comparative study of both protein profiles showed the existence of a pool of 57 proteins that appeared dysregulated in both sham and ischemic animals. These results indicate that the surgical procedure required for intraluminal occlusion of the MCA induces changes in brain protein expression that are not associated with the ischemic lesion. This study highlights the importance of including control sham groups in the experimental design to guarantee that the therapeutic target under study is not affected by the surgical intervention.
HostingRepository	PRIDE
AnnounceDate	2024-08-11
AnnouncementXML	Submission_2024-08-10_16:23:48.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susana Bravo
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-06 09:48:43	ID requested
⏵ 1	2024-08-10 16:23:49	announced

Candamo-Lourido M, Dopico-L, ó, pez A, L, ó, pez-Arias E, L, ó, pez-Amoedo S, Correa-Paz C, Chantada-V, á, zquez MP, Bugallo-Casal A, Del Pozo-Fil, í, u L, P, é, rez-Gayol L, Palomar-Alonso N, Bravo SB, Campos F, P, é, rez-Mato M, Comparative Brain Proteomic Analysis between Sham and Cerebral Ischemia Experimental Groups. Int J Mol Sci, 25(14):(2024) [pubmed]

10.3390/ijms25147538;

submitter keyword: Animal models, stroke., sham, proteomic, experimental design

Susana Belén Bravo López
contact affiliation	Instituto de Investigaciones Sanitarias de Santiago de Compostela
contact email	sbbravo@gmail.com
lab head
Susana Bravo
contact affiliation	FIDIS
contact email	sbbravo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD052899

PRIDE project URI

• PRIDE
  1. PXD052899
     1. Label: PRIDE project
     2. Name: Comparative brain proteomic analysis between sham and cerebral ischemia experimental groups