PXD052886-1

PXD052886 is an original dataset announced via ProteomeXchange.

Title	Cross-sectional study of proteomic differences between moderate and severe psoriasis
Description	Background: Although there is an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic diversity of psoriatic lesions between moderate and severe psoriasis. Objective: To evaluate the proteomic differences between moderate and severe psoriasis and determine biomarkers associated with disease severity. Methods: In this cross-sectional study, we report a proteomic analysis of psoriatic lesions from patients with severe PV (sPV group, n=7) using data-independent acquisition mass spectrometry (DIA-MS), as compared with patients with moderate psoriasis (mPV group, n=8). Results: 173 differentially expressed proteins (DEPs) were significantly differentially expressed between the sPV and mPV groups. Among them, 85 proteins were upregulated, while 88 were downregulated (FC ≥±1.5, P <0.05). Eighteen DEPs were mainly enriched in the IL−17 signalling pathway, Neutrophil extracellular trap formation, Neutrophil degranulation and NF−kappa B signalling pathway, which were associated with psoriasis pathogenesis. Ingenuity pathway (IPA) analysis identified TNF and TDP53 as the top upstream up-regulators, while Lipopolysaccharide and YAP1 were the top potential down-regulators. The main active pathways were antimicrobial peptides and PTEN signalling, while the inhibitory pathways were the neutrophil extracellular trap pathway, neutrophil degranulation, and IL-8 signalling. 4D-parallel reaction monitoring (4D-PRM) analysis suggested that KRT6A were downregulated in sPV.
HostingRepository	iProX
AnnounceDate	2024-06-06
AnnouncementXML	Submission_2024-06-05_22:33:32.696.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiang Dong
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-05 22:31:58	ID requested
⏵ 1	2024-06-05 22:33:33	announced

Dataset with its publication pending

submitter keyword: Proteomics, Psoriasis Vulgaris, 4D-Parallel Reaction Monitoring, Data-Independent Acquisition Mass Spectrometry, Ingenuity Pathway Analysis, Biomarker.

Qiang Dong
contact affiliation	Dermatology Hospital of Zhejiang Province
contact email	QiangDong.edu@outlook.com
lab head
Qiang Dong
contact affiliation	Dermatology Hospital of Zhejiang Province
contact email	QiangDong.edu@outlook.com
dataset submitter

iProX dataset URI