PXD052819-1

PXD052819 is an original dataset announced via ProteomeXchange.

Title	EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration
Description	EPLIN, an actin-binding protein, has been described as both a tumor promoter and tumor suppressor in different cancers. The roles of EPLIN isoforms (α/β) remain largely unknown and could explain these opposing views. We observed distinct EPLIN isoform localization in breast cancer cells; EPLINα is recruited to actin in plasma membrane ruffles and endosomes, while EPLINβ resides on stress fibers. EPLINα localizes to early endosomes in an actin-dependent manner where it interacts with Rab21, an established regulator of β1-integrin endosomal traffic. This supports β1-integrin recycling and cell migration. Using proximity biotinylation (BioID), we identified coronin 1C as an EPLIN proximal protein, which also localizes at Rab21-containing endosomes and controls integrin recycling downstream of EPLINα. EPLINα expression was linked to increased breast cancer cell motility, and high EPLINα-to-EPLINβ ratio correlated with a mesenchymal phenotype in patient samples. Our work identifies previously unknown EPLIN isoform-specific functions relevant to breast cancer and beyond.
HostingRepository	PRIDE
AnnounceDate	2025-08-26
AnnouncementXML	Submission_2025-08-26_01:52:47.018.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Megan Chastney
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-04 03:17:56	ID requested
⏵ 1	2025-08-26 01:52:47	announced
2	2025-08-26 02:22:05	announced	2025-08-26: Updated project metadata.

Dataset with its publication pending

submitter keyword: BioID, breast cancer, LC-MSMS, Human

Johanna Ivaska
contact affiliation	Turku Bioscience Centre, University of Turku, Finland
contact email	johanna.ivaska@utu.fi
lab head
Megan Chastney
contact affiliation	University of Turku
contact email	megan.chastney@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052819
     1. Label: PRIDE project
     2. Name: EPLINα controls integrin recycling from Rab21 endosomes to drive breast cancer cell migration