PXD052797-1

PXD052797 is an original dataset announced via ProteomeXchange.

Title	Increased levels of extracellular matrix proteins in extracellular vesicles from brains of aged mice.
Description	Extracellular vesicles (EVs) have several functions in the brain, serving as a mode of intercellular communication and as a pathway for disposal of cellular debris. While these functions serve to maintain healthy brain function, they may also contribute to diseases affecting the brain. EVs also change with aging of the brain, as levels of some EV subtypes increase at advanced ages in mice. These changes could be caused by aging-related changes such as inflammation, cellular senescence, or impairment of autophagy or mitochondrial function. Aging-related changes in brain EVs might also further aging-related changes, as levels of EVs in plasma may change with age and contribute to aging-related changes in inflammation and cellular metabolism. However, the effects of aging on brain EVs and the potential contribution of EVs to aging-related changes in the brain are not well understood. To address this question, we investigated the levels and protein contents of EVs isolated from brains of 4-, 12-, and 22-month–old C57Bl/6J mice. We detected no changes in EV levels, but observed age-dependent changes in EV proteins. EV fractions from aged (22-month–old) mice contained higher levels of extracellular matrix proteins than EV fractions from young (4-month–old) mice, with similar trends occurring in 12-month–old mice. Specifically, levels of hyaluronan and proteoglycan link proteins (Hapln) 1 and 2 were elevated in EV fractions from aged mice, as were levels of several chondroitin sulfate proteoglycans (CSPGs), which interact with Hapln1 and 2. Analysis of extracellular matrix in several brain regions of aged mice revealed increased immunolabeling for aggrecan, but an overall reduction in labeling with Wisteria floribunda agglutinin, which binds to chondroitin sulfate. These data are consistent with prior studies showing changes to the composition of extracellular matrix in aged brains, which might contribute to age-related cognitive decline. These findings reveal a novel association of EVs with changes in the extracellular matrix of the aging brain.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_18:11:45.391.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD052797
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	james mobley
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-03 10:56:50	ID requested
⏵ 1	2025-05-06 18:11:46	announced

Kaplelach AK, Murchison CF, Kojima K, Mobley JA, Arrant AE, Increased levels of extracellular matrix proteins associated with extracellular vesicles from brains of aged mice. Aging Cell, 24(1):e14359(2025) [pubmed]

10.6019/PXD052797;

10.1111/acel.14359;

submitter keyword: Aging,Extracellular vesicles, Extracellular matrix, LCMS, Mouse Model, Proteomics

James Mobley, Ph.D.
contact affiliation	University of Alabama at Birmingham, IRCP Heersink & CCC O'Neal Proteomics Shared Resource Facility
contact email	mobleyja@uab.edu
lab head
james mobley
contact affiliation	University of Alabama at Birmingham
contact email	mobleyja@uab.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052797
     1. Label: PRIDE project
     2. Name: Increased levels of extracellular matrix proteins in extracellular vesicles from brains of aged mice.