PXD052794 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The effect of Venetin-1 against cyclophosphamide-induced lung toxicity in mice |
Description | Cyclophosphamide (CPAm) is a widely used chemotherapeutic agent that exhibits potent anti-cancer properties but is often associated with debilitating side effects. Despite its efficacy, the management of CPAm-induced toxicities remains a significant clinical challenge. There has been growing interest in exploring complementary and alternative therapies to mitigate these adverse effects in recent years, and this may be a chance for the earthworm-derived preparation, Venetin 1. Its rich composition of bioactive compounds has demonstrated promising pharmacological properties, including anti-inflammatory, antioxidant, and immunomodulatory effects. These properties suggest its potential to counteract the various systemic toxicities induced by CP. To investigate the effect of Venetin 1 on cyclophosphamide-induced toxicity, we conducted a comprehensive study. Mice were administered CPAm for four days, followed by the earthworm preparation in two doses (50 mg/kg and 100 mg/kg b.w). Importantly, the preparation did not cause any side effects in all mice, ensuring the safety of the intervention. We then determined global changes in the proteome using proteomics and quantitative SWATH-MS analysis, a robust and reliable method. This allowed us to identify up- and downregulated proteins in each studied group, providing valuable insights into the mechanism of action of Venetin 1. According to the obtained results, Venetin 1 significantly affected the proteome of mouse lung tissue. It was possible to determine quantitative changes for 400 proteins, and the analysis after administration of Venetin 1 showed a change in the global proteomic profile from upregulated to down-regulated. The stimulating properties of the preparation concerning the complement system have also been confirmed in a separate validation experiment. It has been shown that Venetin 1 has the potential to eliminate the toxic effect of cyclophosphamide in lung tissue. It also initiates regenerative processes, inhibits inflammatory processes, supports autophagy and stimulates the immune system. Further research is required to understand and to fully describe the effects of Venetin 1 . |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-06_18:16:18.759.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paulina Czaplewska |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-06-03 08:07:58 | ID requested | |
⏵ 1 | 2025-05-06 18:16:19 | announced | |
Publication List
10.1038/s41598-024-76143-0; |
Czaplewska P, M, ü, ller M, Musia, ł N, Okr, ó, j M, Felberg-Mi, ę, tka A, Sadowska J, Dudzi, ń, ska W, Lubkowska A, Tokarz-Deptu, ł, a B, Fio, ł, ka M, Preliminary proteomic analysis of mouse lung tissue treated with cyclophosphamide and Venetin-1. Sci Rep, 14(1):25056(2024) [pubmed] |
Keyword List
submitter keyword: Cyclophosphamide, proteomics, lungs, earthworm, SWATH, mass spectrometry, immunosuppression |
Contact List
Paulina Czaplewska |
contact affiliation | Intercollegiate Faculty of Biotechnology University of Gdansk |
contact email | paulina.czaplewska@ug.edu.pl |
lab head | |
Paulina Czaplewska |
contact affiliation | University of Gdansk |
contact email | paulina.czaplewska@ug.edu.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD052794 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD052794
- Label: PRIDE project
- Name: The effect of Venetin-1 against cyclophosphamide-induced lung toxicity in mice