PXD052789-1

PXD052789 is an original dataset announced via ProteomeXchange.

Title	Uracil phosphoribosyltransferase is required to establish a functional cytochrome b6/f complex
Description	Arabidopsis uracil phosphoribosyltransferase (UPP) is an essential enzyme and plants lacking this enzyme are strongly compromised in chloroplast function. Our analysis of UPP amiRNA mutants has confirmed that this vital function is crucial to establish a fully functional photosynthesis as the RIESKE iron sulfur protein (PetC) is almost absent, leading to a block in photosynthetic electron transport. Interestingly, this function appears to be unrelated to nucleotide homeostasis since nucleotide levels were not altered in the studied mutants. Transcriptomics and proteomic analysis showed that protein homeostasis but not gene expression is most likely responsible for this observation and high light (HL) provoked an upregulation of protease levels, including thylakoid filamentation temperature-sensitive 1, 5 (FtsH), caseinolytic protease proteolytic subunit 1 (ClpP1), and processing peptidases, as well as components of the chloroplast protein import machinery in UPP amiRNA lines. Strongly reduced PetC amounts were not only detected by immunoblot from mature plants but in addition in de-etiolation experiment with young seedlings and are causing reduced high light-induced non-photochemical quenching (NPQ) but increased unregulated energy dissipation (NO). Our work suggests that UPP assists in stabilization PetC during the assembly of the Fe-S cluster and targeting to the thylakoid or protects it against proteolytic degradation.
HostingRepository	PRIDE
AnnounceDate	2024-12-09
AnnouncementXML	Submission_2024-12-09_10:33:58.178.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Schwenkert
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	impact HD

Revision	Datetime	Status	ChangeLog Entry
0	2024-06-03 01:34:09	ID requested
⏵ 1	2024-12-09 10:33:59	announced

10.1111/tpj.17036;

Scherer V, Bellin L, Schwenkert S, Lehmann M, Rinne J, Witte CP, Jahnke K, Richter A, Pruss T, Lau A, Waller L, Stein S, Leister D, M, ö, hlmann T, f complex. Plant J, 120(3):1064-1078(2024) [pubmed]

submitter keyword: Chloroplast, Uracil phosphoribosyltransferase,Arabidopsis, high light, protease

Serena Schwenkert
contact affiliation	Biozentrum der LMU München Pflanzenwissenschaften Großhaderner Str. 2-4 82152 Planegg-Martinsried Germany
contact email	serena.schwenkert@lmu.de
lab head
Serena Schwenkert
contact affiliation	LMU Munich
contact email	serena.schwenkert@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD052789
     1. Label: PRIDE project
     2. Name: Uracil phosphoribosyltransferase is required to establish a functional cytochrome b6/f complex